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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
2006
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal. Study conducted according a standardised method.
Reason / purpose:
reference to same study
Reason / purpose:
reference to same study
Qualifier:
according to
Guideline:
other: Methods for Examination of Waters and Associated Materials: 5 Day Biochemical Oxygen Demand (BOD5)
Version / remarks:
2nd edition 1988 (with Dissolved oxygen in waters, Amendment 1988)
Procedure recommended by the Standing Committee of Analysts of the UK Department of the Environment
Principles of method if other than guideline:
The effect of the test substance on the degree of biodegradation of two easily biodegradable substances, glucose and glutamic acid, was also considered to measure the inhibition potential of the substance.
GLP compliance:
not specified
Specific details on test material used for the study:
Composition of test material, percentage of components: Trihexyl(tetradecyl)phosphonium chloride (258864-54-9), >= 95%
Analytical monitoring:
not specified
Vehicle:
no
Test organisms (species):
other: Unknown
Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
5 d
Hardness:
Not specified
Test temperature:
Not specified
pH:
Not specified
Dissolved oxygen:
Not specified
Salinity:
Not specified
Conductivity:
Not specified
Nominal and measured concentrations:
1, 10 and 100 mg/L (nominal)
Reference substance (positive control):
no
Duration:
5 d
Dose descriptor:
NOEC
Effect conc.:
< 1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Inhibition of biodegradation of glucose/glutamate
Details on results:
After 5 days of exposition, the inhibition of glucose/glutamate biodegradation was 100% at concentrations of 10 and 100 mg/L, and of 78% at 1 mg/L.
Therefore, the test material was inhibitory to the microorganisms at all tested concentrations.
Validity criteria fulfilled:
not specified
Conclusions:
The test material was submitted to a test for potential inhibition to degrading microorganisms in a pre-test to a 28-day biodegradation study.
The test material was inhibitory at all tested concentrations. Therefore the NOEC was < 1 mg/L (the 28-day biodegradation study was not conducted because of this result).
Executive summary:

In this study, the biodegradability of the test substance (Trihexyltetradecylphosphonium chloride) was meant to be tested according to a UK guideline ( Methods for Examination of Waters and Associated Materials: 5 Day Biochemical Oxygen Demand (BOD5)). This test includes a screening study to determine the inhibitory properties of the substance before a thorough biodegradation study is run.

The test material was tested concurrently with glucose and glutamate. The degree of inhibition of the degradation of those two reference substances was 100% at 10 and 100 mg/L and of 78% at 1 mg/L. Therefore, the substance was shown to be inhibitory to the microorganisms and the NOEC is < 1 mg/L.

Description of key information

In this study, the biodegradability of the test substance (Trihexyltetradecylphosphonium chloride) was meant to be tested according to a UK guideline ( Methods for Examination of Waters and Associated Materials: 5 Day Biochemical Oxygen Demand (BOD5)). This test includes a screening study to determine the inhibitory properties of the substance before a thorough biodegradation study is run.

The test material was tested concurrently with glucose and glutamate. The degree of inhibition of the degradation of those two reference substances was 100% at 10 and 100 mg/L and of 78% at 1 mg/L. Therefore, the substance was shown to be inhibitory to the microorganisms and the NOEC is < 1 mg/L.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
1 mg/L

Additional information