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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal. Study conducted according to standard methodology. As no analytical monitoring has been performed, the concentration tested cannot be checked. Therefore, this study is considered as reliable with restrictions.
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
(2004)
Deviations:
no
GLP compliance:
not specified
Remarks:
The information available in the publication does not indicate whether or not the laboratory conducted the study under GLP conditions.
Specific details on test material used for the study:
Composition of test material, percentage of components: Trihexyl(tetradecyl)phosphonium chloride (258864-54-9), 96.4%
Analytical monitoring:
not specified
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Daphnia magna Straus 1820 (Cladocera crustacea)
- Age at study initiation (mean and range, SD): <24 h old
- Feeding during test: Not fed
- Method of breeding: continuous and balanced breeding
- Culture water: 'M4 medium', partly renewed once a week
Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
No data
Test temperature:
20 +/- 2°C
pH:
No data
Dissolved oxygen:
No data
Salinity:
Not applicable
Conductivity:
No data
Nominal and measured concentrations:
Nominal concentrations of test material ranged from 0.03 to 1.0 mg/L. These concentrations were selected according a preliminary range-finding screening test.
Details on test conditions:
TEST SYSTEM
- Test vessel: glass test tubes filled with 10 mL of test solution
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2 mL for each daphnid

OTHER TEST CONDITIONS
- Photoperiod: All the tests were conducted in darkness
Reference substance (positive control):
yes
Remarks:
potassium dichromate (K2Cr2O7)
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.053 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
EC50 value is derived from the concentration-response curve using a logistic Hill model . The 95% confidence intervals was estimated using a “bootstrap” simulation method.
EC50 48 h = 0.053 mg/L (95% IC: 0.046–0.054)
Validity criteria fulfilled:
not specified
Conclusions:
Trihexyltetradecylphosphonium chloride was very toxic to daphnids, with a reported EC50 value of 0.053 mg/L (nominal concentration).
Executive summary:

In this study, the acute toxicity of the test substance (Trihexyltetradecylphosphonium chloride) to Daphnia magna was determined in a 48 hour test according to the OECD Guideline No. 202 (2004).

 

The test substance concentrations, selected for the main test, were based on a preliminary range-finding screening test and ranged from 0.03 to 1.0 mg/L. Stock solutions were prepared by introducing directly the required amount of the selected compound in dilution water. 20 animals were tested per concentration. Observations were made on the immobilization rate.

 

In the condition of this study, a 48h EC50 of 0.053 mg/L is obtained. This result is expressed as nominal concentration since no analytical measurement has been performed.

Description of key information

The acute toxicity of Trihexyltetradecylphosphonium chloride to Daphnia magna was investigated in a 48 hour test according to the OECD Guideline No. 202 (2004).

The test substance concentrations, selected for the main test, were based on a preliminary range-finding screening test and ranged from 0.03 to 1.0 mg/L. Stock solutions were prepared by introducing directly the required amount of the selected compound in dilution water. 20 animals were tested per concentration. Observations were made on the immobilization rate.

In the condition of this study, a 48h EC50 of 0.053 mg/L is obtained. This result is expressed as nominal concentration since no analytical measurement has been performed.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
0.053 mg/L

Additional information