Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
3 hour exposure
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Performed to GLP using current guidelines for 2018
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Dried (water-free) sample used
Purity: 92.5%
Physical state/Appearance: greenish-yellow viscous liquid
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The test item was dissolved directly in water
Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
From Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK which treats predominantly domestic sewage.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
pH:
7.3
Dissolved oxygen:
The dissolved oxygen concentrations after 30 minutes contact time in all vessels were above 60% of the dissolved oxygen saturation level of 8.9 mg O2/L.
Nominal and measured concentrations:
Nominal test concentrations of 10, 100 and 1000 mg/L in the range-finding
Nominal test concentrations of 10, 32, 100, 320 and 1000 mg/L in the defnitive test
(three replicates for the 1000 mg/L test concentration).
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
ca. 64 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
ca. 430 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Duration:
3 h
Dose descriptor:
EC100
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Details on results:
No significant toxic effects were shown at the test concentrations of 10 and 32 mg/L
Results with reference substance (positive control):
The positive control gave an EC50 of 6.3 - 10 mg/L and is considered valid
Reported statistics and error estimates:
Full statistical analysis was not possible, but the results provide sufficient information to indicate the level of inhibition
Validity criteria fulfilled:
yes
Conclusions:
The effect of the test item on the respiration of activated sewage sludge micro-organisms gave a 3-Hour EC50 value of 430 mg/L.
The NOEC after 3 hours exposure was 32 mg/L.

Description of key information

The effect of the test item on the respiration of activated sewage sludge micro-organisms gave a 3-Hour EC50 value of 430 mg/L.

The NOEC after 3 hours exposure was 32 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
430 mg/L
EC10 or NOEC for microorganisms:
32 mg/L

Additional information