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EC number: 946-856-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06.11.2018 - 07.01.2019
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April 2004
- GLP compliance:
- yes (incl. QA statement)
Test material
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Photometric analysis of the test item concentration in the test solutions was performed to
determine exposure concentration and stability of the test item during the test. One analytic sample
of ca. 10 mL was taken from each of the two additional vessels without daphndis of treatment A, C,
E and NC at the start of the experiment and after 24 h and 48 h. The samples were filled into 15
mL falcons and stored in the fridge (5°C ± 3°C) until measurement.
One analytic sample of the treatments A, C, E and NC from 0, 24 and 48 h was analyzed within ≤ 7
d after sampling. The storage stability of the analytical samples was verified over a period of 7 d
(Validation Study No. 1391, Hydrotox GmbH). The remaining samples were stored as retain
samples in the fridge (5 °C ± 3 °C) until finalization of the study.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- five nominal test item concentrations of 18.75 – 300 mg/L active substance were used.
Test organisms
- Test organisms (species):
- other: Daphnia magna Strauss
- Details on test organisms:
- The test organism Daphnia magna Straus originates from the clone breeding of the Federal
Environment Agency, department IV 2.4, Berlin, and has been cultivated at Hydrotox GmbH since
October 2012. The daphnids are cultured at 20 ± 2 °C with 16 h light : 8 h dark and placed into
fresh Elendt M4 medium twice a week. They are kept at approx. 10 animals / 200 mL and fed on
workdays with Desmodesmus subspicatus algae corresponding to 0.1 mg C × daphnid-1 × day-1.
The test organisms used were 2.45 – 22.45 h old at the start of the test.
Study design
- Test type:
- static
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- The swimming capability of the daphnids was assessed after 24 h and 48 h exposure.
Test conditions
- Test temperature:
- 20.2 – 21.9 °C
- pH:
- 7.5 - 7.8
- Dissolved oxygen:
- 8.0 - 8.4 mg/L
- Conductivity:
- conductivity (684 μS/cm)
- Nominal and measured concentrations:
- Nominal: 24.88, 37.32, 55.98, 83.96 and 125.94 mg/L
- Details on test conditions:
- • The pH was 7.6 – 7.6 in the control and 7.5 – 7.8 in the test item treatments (recommended: 6 –
9; Table 3).
• The dissolved oxygen concentration was 8.0 – 8.4 mg/L in the control and 8.2 – 8.3 mg/L in the
test item treatment (required: ≥ 3 mg/L; Table 3).
• The temperature during the exposure was 20.2 – 21.9 °C (required: 18 – 22 °C, constant within
± 1 °C).
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 129.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC10
- Effect conc.:
- 38.38 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 44.44 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 29.63 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 23.69 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Remarks on result:
- not determinable
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- <= 19.75 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- <= 19.75 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Effect concentrations
EC50 (24h) 129.25 mg/L
EC50 (48h) 23.69 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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