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EC number: 200-854-6 | CAS number: 75-25-2
Mortality among water flea control populations never exceeded 10% in any test (study was part of a series of tests on a number of compounds).
The toxicity of the test substance to Daphnia magna was investigated using procedures presented in: "Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians" (U.S. EPA 1975).
Five daphnids were randomly placed in 250 mL beakers containing 150 mL of test solution at up to five to eight nominal concentrations. Exposures were undertaken in triplicate. A negative control consisting of the same dilution water, test conditions, and test organisms, but containing no test substance was maintained concurrently. During the tests, the dissolved oxygen concentration, pH and temperature of test solutions were measured at the initiation and termination of the toxicity tests in the high, middle and low test concentrations and controls.
Observations of test populations were made at 24 and 48 hours of exposure and any mortalities were recorded.
The 48-hour EC50 of the test substance to Daphnia magna was 46 mg/L based on mortality. The 48-hour NOEC was <7.8 mg/L.
Toxicity of the test substance to Daphnia magna.
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