Absolute of Cistus ladaniferus (Cistaceae) obtained from cistus concrete by ethanol extraction

Density

Link to relevant study record(s)

Reference

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14-17 May 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was conducted according to an internationally recognised method, and under GLP. The substance is considered to be adequately characterised. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Version / remarks:

2012

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Version / remarks:

2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

GLP certificate (inspected on 15 to 16 November 2017 / signed on 15 May 2018)

Type of method:

pycnometer method

Remarks:

using a replacement liquid

Key result

Type:

density

Density:

1.078 g/cm³

Temp.:

20 °C

Remarks on result:

other:

Remarks:

mean of two independent determinations

Key result

Type:

relative density

Density:

1.078 other: unitless

Temp.:

20 °C

Remarks on result:

other:

Remarks:

mean of two independent determinations

Pre-test

At 80°C, the test item melted to a brown, viscous, sticky flowable liquid. 

After cooling down, it was doused with deionised water. As the test item stayed at the bottom and did not dissolve, deionised water was considered applicable as replacement liquid.

Table 4.4/1: Measured Values

First Determination				Error rel.	Error abs.
Temperature	20.0	°C			0.1
Density of water	0.998203	g/cm^3		2.00%	0.0002
Weighing #	m1	m2	m3	m4	m5
Item	Pycn. empty	Pycn. + H2O	Pycn. + repl.liquid	Pycn. + test item	Pycn. + test item + repl. liquid
Mass in g	32.5645	57.5928	57.5939	38.7226	58.0404
Temperature in °C	20.0	19.8	20.0	20.0	20.0
Volume of Pycnometer	25.0734	cm^3
Density of Replacement liquid	0.9982	g/cm^3		Error rel.	Error abs.
Density of Test Item	1.0763	g/cm^3		0.0355%	0.0004
Second Determination				Error rel.	Error abs.
Temperature	19.8	°C			0.1
Density of water	0.998203	g/cm^3		2.00%	0.0002
Weighing #	m1	m2	m3	m4	m5
Item	Pycn. empty	Pycn. + H2O	Pycn. + repl.liquid	Pycn. + test item	Pycn. + test item + repl. liquid
Mass in g	32.5641	57.5893	57.5957	38.8418	57.9928
Temperature in °C	19.8	19.8	20.0	20.0	20.2
Volume of Pycnometer	25.0703	cm^3
Density of Replacement liquid	0.9985	g/cm^3		Error rel.	Error abs.
Density of Test Item	1.0797	g/cm^3		0.0408%	0.0004

Results of the single determinations with the respective error as well as the mean value and its standard deviation are presented in the following tables:

Table 4.4/2: Results of Single Determinations

 

Determination	Density in g/cm^3	Error of Determ. in g/cm^3
1	1.0763	0.0004
2	1.0797	0.0004

 

Table 4.4/3 Results – Mean Values

 

Parameter	Value	Unit	Standard Deviation	Relative Standard Deviation
Volume of Pycnometer	25.07	cm^3	0.0022	0.01%
Density of Test Item	1.0780	g/ cm^3	0.0024	0.22%
Density of Test Item	1078.0	kg/m^3	2.4	0.04%
Relative Density of Test Item (D20/4)	1.0780		0.0024	0.04%

The relative density of the test item has the same number value, but no unit, assuming that relative density is given as the ratio of test item density to density of water at 4°C, having a standard density of 0.999975 g/cm3 (source: CRC Handbook of Chemistry and Physics - 90th edition / Section 6 - Fluid properties - p.6 -7).

The density of the test item was calculated as 1.0780 ± 0.0004 g/cm³at 20.0 ± 0.2 °C, which is the mean of two independent determinations ± the standard deviation. Standard deviation was 0.0024 g/cm³ shows good correlation of the two determinations. Therefore, the study is considered valid.

Conclusions:

The density of the test substance was calculated as 1.0780 ± 0.0004 g/cm3 at 20.0 ± 0.2 °C and the relative density is determined as D20/4 = 1.0780
Denser than water

Executive summary:

The density of the test substance was measured under GLP according OECD 109 / EU A3 guideline, using the pycnometer method with a replacement liquid.

Two determinations were performed and the mean value was retained.

This result was then converted to relative density towards water at 4°C.

Mean density: 1.0780 g/cm³at 20.0 ± 0.2 °C

Mean relative density (D20/4) : 1.0780

Description of key information

The density of the test item was measured as 1.0780 ± 0.0004 g/cm3 at 20.0 ± 0.2 °C and the relative density is determined as D20/4 = 1.0780

Key value for chemical safety assessment

Relative density at 20C:

1.078

Additional information

A fully reliable experimental study, conducted under GLP according to OECD 109 /EU A3 guideline, using pycnometer method with a replacement liquid, is available. It is considered as a key study, and the result is retained as key data.