Fatty acids, rape-oil, hydrogenated, reaction products with diethylenetriamine, di-Me sulfate-quaternized

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From June 24, 2010 to August 19, 2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Remarks:

Spectrometry

Details on sampling:

Throughout the study (at 0, 24, 48, 72 and 96 h)

Vehicle:

no

Details on test solutions:

The test solution was prepared by directly dissolving appropriate amount of test substance in dilution medium and then facilitating its dispersion by ultrasonication for 30 min.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Source: Institute of hydrobiology, Chinese academy of sciences
Acclimation: 7 days
Photoperiod: 16 hours light
Oxygen concentration: > 80%
Diet: daily

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

119 - 122 mg CaCO3/L

Test temperature:

23.0 - 23.1

pH:

7.00 - 7.63

Dissolved oxygen:

96 - 103% of the air saturation

Salinity:

-

Conductivity:

-

Nominal and measured concentrations:

0 (negative control) to 50 mg/L

Details on test conditions:

Based on a range-finding test, a definitive test was performed.
Three replicates were assigned for each group
The number of fish was 10
During the definitive test, mortality, inactivity, strange swimming pattern and other abnormal behaviours were recorded.
pH, dissolved oxygen and temperature were recorded as well throughout the experiment.

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

29.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: analysed concentration of 27.7 mg/L

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

20 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: analysed concentration of 19.2 mg/L

Details on results:

All fish in the control group were alive and appeared normal. The treated fishes were observed to be lying on side or back and abnormally swift swimming with the test concentration no less than 30 mg/L. Fishes treated with 50 mg/L test substance were also observed to be tumbling during swimming.

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, the 96 h LC50 and NOEC (in Danio rerio) of the test substance were determined to be 29.4 and 20 mg/L (nominal), corresponding to 27.7 and 19.2 mg/L (measured concentrations), respectively.

Executive summary:

A study was conducted to determine the short-term toxicity of the test substance, 'di-C18-22 AAEMIM-MS' (active: 100%), to fish according to OECD Guideline 203, in compliance with GLP. Based on a range-finding test, a definitive test was performed with test substance concentrations of 0 to 50 mg/L under static conditions. Three replicates of 10 Danio rerio were assigned for each group. During the definitive test, mortality, inactivity, strange swimming pattern and other abnormal behaviours were recorded. pH, dissolved oxygen and temperature were measured as well throughout the experiment. Test substance concentrations were analysed by spectrometry at 0, 24, 48, 72 and 96 h and showed a good linearity when increasing the dose. All fish in the control group were alive and appeared normal. The treated fishes were observed to be lying on side or back and abnormally swift swimming with the test concentration no less than 30 mg/L. Fishes treated with 50 mg/L test substance were observed to be tumbling during swimming. Under the study conditions, the 96 h LC50 and NOEC of the test substance were determined to be 29.4 and 20 mg/L (nominal), corresponding to 27.7 and 19.2 mg/L (measured concentrations), respectively (Key Lab., 2010).

Description of key information

Based on the results of the study, the 96 h LC50 and NOEC of the test substance, di-C18-22 AAEMIM-MS, for toxicity to fish were determined to be 29.4 and 20 mg/L (nominal), corresponding to 27.7 and 19.2 mg/L (measured concentration), respectively.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

27.7 mg/L

Additional information

A study was conducted to determine the short-term toxicity of the test substance, 'di-C18-22 AAEMIM-MS' (active: 100%), to fish according to OECD Guideline 203, in compliance with GLP. Based on a range-finding test, a definitive test was performed with test substance concentrations of 0 to 50 mg/L under static conditions. Three replicates of ten Danio rerio were assigned for each group. During the definitive test, mortality, inactivity, strange swimming pattern and other abnormal behaviours were recorded. pH, dissolved oxygen and temperature were measured as well throughout the experiment. Test substance concentrations were analysed by spectrometry at 0, 24, 48, 72 and 96 h and showed a good linearity when increasing the dose. All fish in the control group were alive and appeared normal. The treated fishes were observed to be lying on side or back and abnormally swift swimming with the test concentration no less than 30 mg/L. Fishes treated with 50 mg/L test substance were observed to be tumbling during swimming. Under the study conditions, the 96 h LC50 and NOEC of the test substance were determined to be 29.4 and 20 mg/L (nominal), corresponding to 27.7 and 19.2 mg/L (measured concentrations), respectively (Key Lab., 2010).