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EC number: 201-132-3 | CAS number: 78-67-1
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,2'-dimethyl-2,2'-azodipropiononitrile
- EC Number:
- 201-132-3
- EC Name:
- 2,2'-dimethyl-2,2'-azodipropiononitrile
- Cas Number:
- 78-67-1
- Molecular formula:
- C8H12N4
- IUPAC Name:
- 2,2'-dimethyl-2,2'-azodipropiononitrile
- Details on test material:
- - Name of test material (as cited in study report): 2,2’-Azobis(2-methylpropionitrile)
- Analytical purity: 99 %
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Sludge was taken from 10 different sites (municipal STPs, industrial STPs, lakes and rivers) in Japan
- Concentration of sludge: 30 mg/L - Duration of test (contact time):
- 28 d
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 7
- Sampling time:
- 28 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test substance was not ready biodegradable under the test conditions within 28 days.
- Executive summary:
Materials and methods
A Modified MITI Test (I) was conducted according to OECD Guideline 301 C over a period of 28 days.
Results and discussion
No biodegradation of test substance was observed during a 28 d incubation period. The test item is therefore considered to be not ready biodegradable.
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