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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1988
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Version / remarks:
Annex EEC Directive 84/449.
Deviations:
yes
Remarks:
see "Principles of method other than guideline"
Principles of method if other than guideline:
For the main test 5 fish were used per vessel. The total number of fish per concentration was 10 which is in accordance with the test guidelines. Becasue test material appeared to hydrolyse in aqueous solution it was decided to renew the test media every 24 hours
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
(ethyl-3-oxobutanoato-O'1,O'3)(2-dimethylaminoethanolato)(1-methoxypropan-2-olato)aluminium(III), dimerised
EC Number:
402-370-2
EC Name:
(ethyl-3-oxobutanoato-O'1,O'3)(2-dimethylaminoethanolato)(1-methoxypropan-2-olato)aluminium(III), dimerised
Cas Number:
149057-70-5
Molecular formula:
C14H28AlNO6
IUPAC Name:
octaaluminium(3+) octakis((2Z)-4-ethoxy-4-oxobut-2-en-2-olate) octakis(1-methoxypropan-2-olate) octakis(2-(dimethylamino)ethan-1-olate)
Test material form:
liquid
Details on test material:
purity not specified, batch 25/2/87R

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
The samples taken from the test media (=100 ml) were treated as follows: 10 ml of a sample.was stirred vigorously with 1 ml internal standard (C1e) solution in hexane (0.43 mg C1e/ ml hexane, LiChrosolv, Merck, FRG).

Test solutions

Vehicle:
yes
Remarks:
Deutch Standard Water (DSW)
Details on test solutions:
Deutch

Test organisms

Test organisms (species):
Poecilia reticulata

Study design

Test type:
semi-static
Water media type:
other: DSW
Total exposure duration:
96 h

Test conditions

Hardness:
11.7 DH
pH:
8.2 +/-0.2

Results and discussion

Effect concentrations
Key result
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
>= 0 - <= 560 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Test material was investigated for acute effects in Poecilia reticulata.

A6/l/0 appeared to adsorb strongly to glass material and was hardly soluble in Dutch Standard Water (DSW).

The fish were exposed for 96 hours to nominal concentrations ranging from 56 to 1000 mg A6/1/0/litre of Deutch Standard Water (DSW), forming a geometric progression with a factor of 1.8.

100%  Mortality was observed at the nominal ncentration of 1000 mg/1 after 24 hours of exposure, whereas at 560 mq/L all fish survived, but had increased pigmentation and showed decreased   swimming. These effects disappeared during further exposure.

No effects were seen at the nominal concentrations of 320 mg/1 and lower.

Calculation of the actual concentration of A6/l/0 in DSW was based on determination of the concentrations of the hydrolysis product butoxyethylacetoacetate using a gas chromatographic method. No dectable concentrations of butoxyethylacetoacetate were found in sample from 560 mg/L, whereas the actual concetration of test material calculated in sample of 1000mg/L at 24hours was 21mg/L.

Hence, the nominal calculated 96h-LC50 for fish exposed to A6/l/0 appeared to be between 560 and 1000 mg/1, whereas the actual 96b-LC50 was estimated to be < 60 mg/1. The nominal No Observed Effect Concentration (NOEC) was 560 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The nominal No Observed Effect Concentration (NOEC) was 560 mg/L.
Executive summary:

In an acute toxicity study in poecilia reticulata according to method C1 of EEC Directive 84/449, the nominal No Observed Effect Concentration (NOEC) was 560 mg/L.