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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

EC50 = 100 mg/l (based on nominal concentration)

Key value for chemical safety assessment

Additional information

In the acute immobilization test with Daphnia magna (STRAUS), the effects of the test item were determined at the test facility according to OECD guideline 202.

The study was conducted under semi-static conditions over a period of 48 hours with the limit concentration of 100 mg/L of the test item.The test solution was shaken manually until the solution was visually clear. The limit concentration was colorless and visually clear throughout the exposure.

Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to the limit concentration and the control.

The concentration of Cobalt, component of the test item, was analytically verified via spectrophotometric analysis in the fresh media of the limit concentration and in the control at the start of the exposure and at the renewal of the test solutions (0 and 24 hours) and in the 24-hours old media at the renewal and at the end of the test (24 and 48 hours).

The measured concentration of Cobaltat the start of the exposure (0 and 24 hours) and at the end of the exposure (24 and 48 hours) was between of 5 and 6% of the nominal concentration. The geometric mean measured concentration of Cobalt was 0.754 mg/L.

Since the recoveries of the measured Cobalt concentration were not within ± 20% of the nominal concentration,the effect concentrations are based on geometric mean measured concentration of Cobalt. Additionally, the effect levels are given based on the nominal concentration of the test item.

The validity criteria of the test guidelines were fulfilled.

In the limit concentration 100 mg/Lof the test item, which corresponds to the geometric mean measured concentration of 0.754 mg/L of the component Cobalt, no effects on Daphnia magna were observed.