Reaction product of mixed inorganic base and acid resulting in dipotassium hydroxytetrafluoro triborontrioxide, dipotassium tetrahydroxy tetraboronpentaoxide dehydrate, potassium fluoride, potassium tetrafluroborate, water in paste form

Long-term toxicity to fish

Administrative data

Endpoint:

fish short-term toxicity test on embryo and sac-fry stages

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Peer-reviewed study, non-standard method, in conjunction with boron-depleted fish

Data source

Reference

Reference Type:

publication

Title:

The Response of Trout and Zebrafish Embryos to Low and High Boron Concentrations Is U-Shaped

Author:

Rowe, RI, C Bouzan, S Nabili and CD Eckhert

Year:

1998

Bibliographic source:

Biological Trace Element Research Vol. 66, pp261-270

Materials and methods

Principles of method if other than guideline:

Chronic toxicity was examined in zebrafish utilizing a 96-h exposure. This encompassed the period from fertilization until the fish were free feeding.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Boric acid

EC Number:

233-139-2

EC Name:

Boric acid

Cas Number:

10043-35-3

Molecular formula:

H3BO3

IUPAC Name:

Boric acid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Source: From a parental stock that was maintained for 6 mo in ultrapure water containing 46 µmol B/L

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Nominal and measured concentrations:

0, 1.2, 9.2, 18.5, 37, and 74.0 mmol B/L

Details on test conditions:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The solution used to test zebrafish contained CaCl2.2H2O, 79.9 mmol/L; MgSO4.7H2O, 20 mmol/L; KCl, 3 mmol/L; and NaHCO3, 30 mmol/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LOEC

Effect conc.:

99.36 mg/L

Conc. based on:

element

Basis for effect:

mortality

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

13 mg/L

Conc. based on:

element

Basis for effect:

mortality

Reported statistics and error estimates:

The effects of B were evaluated using a 1- and 2-way ANOVA and the Student Newman-Keuls test. If heterogenous variance was evident, data were treated to arcsin or reciprocal transformation prior to evaluation using the Kruskal-Wallis One Way Analysis of Variance on Ranks. A p value < 0.05 was considered statistically significant