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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
Deviations:
no
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Deviations:
no
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
sewage, predominantly domestic, non-adapted
Details on inoculum:
- Expiration of a biological laboratory activated sludge plant (Wupperverband)
- Use of prescribed volume of the inoculum
Duration of test (contact time):
28 d
Initial conc.:
36.4 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance:
aniline
Parameter:
% degradation (O2 consumption)
Value:
21
Sampling time:
28 d
Results with reference substance:
Kinetic of reference substance (% degradation):
37 after 5 days
30 after 15 days
100 after 28 days
Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
Within 28 days, a degradation rate of 21 % was determined. The test item is considered to be "Not readily biodegradable". The reference compound aniline showed 100 % degradation after 28 days.
Executive summary:

Within 28 days, a degradation rate of 21 % was determined. The test item is considered to be "Not readily biodegradable".

Description of key information

Not readily biodegradable

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

Biodegradation in water of the substance was evaluated by screen tests.

The test substance is considered as not readily biodegradable.