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EC number: 205-305-4 | CAS number: 137-66-6
The possible eye hazard potential of Ascorbyl palmitate was tested through topical application on two tissues for 6 hours. A positive and a negative control were tested concurrently, each on two individual tissues.
The positive control had a mean cell viability of 41% after 6 hours ± 15 minutes exposure. The absolute mean OD570 (optical density at 570 nm) of the negative control tissues was within 0.8 and 2.5. The standard deviation value of the percentage viability of two tissues treated identically was less than 10%, indicating that the test system functioned properly.
Ascorbyl palmitate did interact with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). In addition to the normal procedure, two freeze-killed tissues treated with test item and one freeze-killed negative control treated tissue were used for cytotoxicity evaluation. The non-specific reduction of MTT by Ascorbyl palmitate was 17% of the negative control tissues. The ODs of the test item treated viable tissues was corrected using the OD of the freeze-killed tissues.
Eye hazard potential is expressed as the remaining cell viability after exposure to the test item. The relative mean tissue viability obtained after 6 hours ± 15 minutes treatment with Ascorbyl palmitate compared to the negative control tissues was 33%. Since the mean relative tissue viability for Ascorbyl palmitate was below 60% after 6 hours ± 15 minutes treatment it is considered to be potentially irritant or corrosive to the eye.
Finally, it is concluded that this test is valid and that Ascorbyl palmitate is potentially irritant or corrosive in the EpiOcular™ test under the experimental conditions described in this report.
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