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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
25th june 2002 - 27th june 2002
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods
Qualifier:
equivalent or similar to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
not specified explicitly in text
GLP compliance:
not specified
Analytical monitoring:
no
Details on sampling:
- Concentrations: 0 and 100 mg/L
Vehicle:
yes
Details on test solutions:
prepared in ISO , prepared in milli-RO water
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
not specified
Test temperature:
19.9°C
pH:
7.7 - 8.0
Dissolved oxygen:
7.8 - 9.1 mg/L
Salinity:
not specified
Conductivity:
not specified
Nominal and measured concentrations:
nominal 0 and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Type (delete if not applicable): closed
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
Reference substance (positive control):
not specified
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
one daphnid observed trapped at the surface of the test solutions. these daphnids were re-immersed in the respective solutions before scoring of mobility
Results with reference substance (positive control):
not specified
Reported statistics and error estimates:
not specified
Validity criteria fulfilled:
not specified
Conclusions:
Under the conditions of the the study, 8135C did not induce acute immobilisation of daphnia magna in a WAF loaded at 100 mg/L after 48 hours of exposure. The 48h EC50 was > a WAF loaded at 100mg/L.
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
24th june 2003 - 26th june 2003
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
not specified
Specific details on test material used for the study:
Pigment red 81:5/4 (silicomolybdic acid salt of C.I. 45160:4)
Red powder
Batch: pigment red 81:5/4
Analytical monitoring:
no
Details on sampling:
- Concentrations: 0.01, 0.1, 1, 10 mg/L
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solutions were prepared in acetone at a concetration of a factor of 10,000 higher than the final test solutions
- Eluate: 0.1mL of the stock solution was added per litre of test medium
- Differential loading: water accommodated fractions
- Controls: contain same amount of solvent as test item
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
not specified
Test temperature:
20.2 - 21.0°C
pH:
7.6 - 7.8
Dissolved oxygen:
remained above 7mg/L
Salinity:
not specified
Conductivity:
not specified
Nominal and measured concentrations:
nominal 0.01, 0.1, 1, 10 mg/L
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
< 0.01 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
none specified
Validity criteria fulfilled:
not specified
Conclusions:
The 48h EC50 was below 0.01mg/L
According to EEc directive 98/98, pigment red 81:5/4 should be classified as highly toxic to aquatic organisms
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
24th june 2003 - 26th june 2003
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
not specified
Specific details on test material used for the study:
pigment red 81:5/3
silicomolybdic acid salt of C.I 45160:4
Analytical monitoring:
no
Details on sampling:
concentrations: 0.01, 0.1, 1, 10 mg/L
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solutions prepared in acetone
- Eluate: water acommodated fractions
- Controls: yes, concurrent without test item
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
not specified
Test temperature:
19.3 - 20.6°C
pH:
7.7 - 7.9
Dissolved oxygen:
remained above 7 mg/L
Salinity:
not specified
Conductivity:
not specified
Nominal and measured concentrations:
nominal: 0.01, 0.1, 1, 10 mg/L
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
< 0.01 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
none specified
Reported statistics and error estimates:
not specified
Validity criteria fulfilled:
not specified
Conclusions:
In conclusion the 48h EC50 was below 0.01 mg/L.
According to EEC directive 98/98, pigment red 81:5/3 should be classified as highly toxic to aquatic organisms

Description of key information

Three non-GLP screening test studies have been performed to assess the possible toxicity of the test

substance to fresh water crustaceans.

No analytical information is available to verify the concentration of the test substance in solution.

As the test substance is very poorly water soluble it is proposed to conduct a long term aquatic toxicity

test to fulfil this endpoint as indicated in the REACH guidance

Key value for chemical safety assessment

Additional information