Registration Dossier
Registration Dossier
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EC number: 281-984-0 | CAS number: 84082-36-0 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Medicago sativa, Leguminosae.
Endpoint summary
Administrative data
Description of key information
Algal growth inhibition study (72 hours)
NOEL: 100 mg/L loading rate WAF (growth rate)
NOEL: 32 mg/L loading rate WAF (yield)
EL50: >100 mg/L loading rate WAF (growth rate and yield)
Acute immobilization test in daphnids:
NOEL (24 and 28 hours): 100 mg/L loading rate WAF
EL50: >100 mg/L loading rate WAF
Additional information
Algal growth inhibition test (OECD TG 201) and 48-hour immobilization test in Daphnia magna (OECD TG 202) were conducted to assess the acute aquatic toxicity of Alfalfa, ext.
Due to the low aqueous solubility and complex nature of the test item for the purposes of both tests, the test item was prepared as a Water Accommodated Fraction (WAF).
In the algal growth inhibition study conducted on Pseudokirchneriella subcapitata, there were no abnormalities detected in any of the control or test cultures.
The NOEL values for growth rate and yield were determined at 100 and 32 mg/L loading rate as WAF after 72 hours of exposure to Alfalfa, ext., respectively. The effective loading causing 50% response (EL50) was determined at >100 mg/L loading rate WAF for growth rate and yield.
In the acute immobilization test in Daphnia magna, Alfalfa, ext. there was no immobilization in 20 tested daphnids and no sub-lethal effects were observed during the study period.
The NOEL after 24 and 48 hours of exposure was determined at 100 mg/L loading rate WAF. In addition, the effective loading causing 50% response (EL50) was determined at >100 mg/L loading rate WAF.
Based on the experimental data from the short-term aquatic studies on freshwater algae and daphnids, Alfalfa, ext. does not meet the GHS criteria classification as Acute aquatic hazard category 1.
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