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EC number: 451-620-7 | CAS number: 352230-22-9
Table: Effect of the test substance on mortality of Daphnia magna
48-hour EL50: >100 mg/L (nominal, WAF loading rate) (highest concentration tested); effects of the submission substance (CAS 352230-22-9) on mortality of Daphnia magna.
A 48-hour EL50 value of >100 mg/L (nominal, WAF loading rate) (highest concentration tested) has been determined for the effects of the submission substance (CAS 352230-22-9) on mortality of Daphnia magna, in accordance with OECD Guideline 202 (Daphnia sp., Acute immobilisation Test).
The study reports an analytically determined test substance concentration of <0.7 µg/L (<LOQ, mean measured). This value reflects the low water solubility of the test substance, but losses may also have occurred as a result of filtering of the test solution prior to addition of test organisms, and the potential for adsorption of the test substance onto the filter matrix.
Each constituent of the test substance has a slow hydrolysis half-life at pH 7 (predicted to be >200 to >630 hours). However, the hydrolysis rate greatly increases with higher pH; 2 to 5.3 hours at pH 9. This test was conducted at pH 7.7 -7.8. Some hydrolysis is likely to have occurred in the timescale of the test. In view of the test media preparation method and the static exposure regime it is likely that the test organisms were exposed predominantly to the parent substance at the beginning of the test, and to a mixture of the parent substance and the hydrolysis products of each constituent of the tested substance towards the end of the test.
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