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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2012
Report Date:
2012

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
yes
Remarks:
see 'Principles of method if other than guideline'
Qualifier:
according to
Guideline:
EU Method C.3 (Algal Inhibition test)
Deviations:
yes
Remarks:
see 'Principles of method if other than guideline'
Principles of method if other than guideline:
The concentration of solvent in the treatments was 1.0 mL/L and therefore higher than stated in the guideline. As no inhibition was observed in the solvent control, this deviation was stated as uncritical. Additionally it was proven in several previously conducted experiments that a concentration of 1.0 mL/L acetone is uncritical for algal growth over a period of 72 hours.
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): (1R)-1-[(4R,4aR,8aS)-2,6-bis(4-chlorophenyl)tetrahydro[1,3]dioxino[5,4-d][1,3]dioxin-4-yl]ethane-1,2-diol
- Expiration date of the lot/batch: December 2012
- Storage condition of test material: Room Temperature: (20 ± 5°C); Keep away from humidity

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
>= 0.35 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
other: growth rate, cell number and biomass
Remarks on result:
other: test substance showed no algal cell growth inhibition up to its limit of solubility in water
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 0.35 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
other: growth rate, cell number and biomass
Remarks on result:
other: test substance showed no algal cell growth inhibition up to its limit of solubility in water

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The following results for the test item were determined:

Endpoint NOEC LOEC EC50
Growth Rate ≥ 0.35 mg/L > 0.35 mg/L > 0.35 mg/L
AUC ≥ 0.35 mg/L > 0.35 mg/L > 0.35 mg/L
Yield ≥ 0.35 mg/L > 0.35 mg/L > 0.35 mg/L

[0.35 mg/L = limit of solubility in test medium]

The test substance showed no algal cell growth inhibition up to its limit of solubility in water.