Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

EC50 (30 minutes) > 100 mg/l (nominal)

Key value for chemical safety assessment

EC50 for microorganisms:
100 mg/L

Additional information

Toxicity of the test item to bacteria was evaluated in an experimental study according to the OECD Guideline 209 (1984). 200 ml of aerobic activated sludge, previously collected from a domestic waste water treatment plant, was combined with either the test item (at final concentrations of 3.2, 10, 32, 50 and 100 mg/l test item) or reference substance 3,5-dichlorophenol (at final concentrations of 1.0, 3.2, 10, 32 and 50 mg/l), and with bidistilled water to make 500 ml final volume. Initial pH was recorded and the flasks were kept aerated using compressed air at an air-flow rate of 0.2 l/minute. Oxygen consumption (mg O2/l min.) was monitored for 30 minutes, and the percentage of inhibition was recorded at the end of this time.

The respiration rate of aerobic waste water bacteria was not inhibited (-2.1 % to -5.6 %) when exposed to test item concentrations between 3.2 to 100 mg/l. The EC50 (30 minutes) was therefore determined to be over 100 mg/l. The EC50 (30 minutes, ) for the reference compound, 3,5 -dichlorophenol, which was tested under the same conditions as the test item, was found to be 19.8 mg/l and therefore lies within the range of 5 - 30 mg/l, the range recommended by the test guidelines.