reaction product of: C.I. Leuco Sulfur Black 1 with (3-chloro-2-hydroxypropyl)trimethylammonium chloride

Short-term toxicity to aquatic invertebrates

Description of key information

EC50 (48 hours, Daphnia magna) = 49 mg/l (mean measured)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

49 mg/L

Additional information

The toxicity of the test item to invertebrates was evaluated in an experimental study on Daphnia magna according to the OECD Guideline 202 (1984) and the EU Method C.2 (1992). Four groups of 5 Daphnia per flask were exposed to various concentrations of the test item in aqueous test media for 48 hours in a static test under defined conditions, and were monitored daily for imobilisation. The nominal concentrations of test item were 9.2, 20, 42, 92 and 200 mg/l. Flasks were monitored for oxygen concentration, pH level, and behaviour of the test item in test media at the beginning and end of the study period.

In the test media the measured test substance concentrations at the start of the test ranged from 81 % to 98 % of the nominal values. During the test period of 48 hours a slight decrease of test substance concentration in the test media was determined. The mean measured test concentrations (calculated as the average over all measurements per test concentration) varied in the range of 76 % to 96 % of the nominal values. Therefore, the reponed biological results are related to the mean measured test substance concentrations.

Strong colouration of the test media and the Daphnia was observed, caused by the test item. Dissolved oxygen concentration ranged from 8.4 to 10.2 mg/l during the study period. Based on the observations, the EC50 (48 hours) was determined to be 49 mg/l test item at a 95 % confidence interval from 33 to 74 mg/l.