Dihydrogen bis[P,P-dioctyl diphosphato(2-)-O'',O''''][hydroxyacetato(2-)-O1,O2]titanate(2-), branched and linear

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23 - 25 May 2018

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Remarks:

The study was conducted in full compliance with GLP guidelines with one exception. For more details please refer to field "Any other Information on Material and Methods incl. tables".

Test material

Test material information

1

Reference substance name:

Dihydrogen bis[P,P-dioctyl diphosphato(2-)-O'',O''''][hydroxyacetato(2-)-O1,O2]titanate(2-), branched and linear

EC Number:

271-571-3

EC Name:

Dihydrogen bis[P,P-dioctyl diphosphato(2-)-O'',O''''][hydroxyacetato(2-)-O1,O2]titanate(2-), branched and linear

Cas Number:

68585-64-8

Molecular formula:

not applicable (UVCB substance)

IUPAC Name:

({bis[(2-ethylhexyl)oxy]phosphoryl}oxy)[(2-{[({bis[(2-ethylhexyl)oxy]phosphoryl}oxy)(hydroxy)phosphoryl]oxy}-4-oxo-1,3-dioxa-2-titanacyclopentan-2-yl)oxy]phosphinic acid; {[({bis[(2-ethylhexyl)oxy]phosphanyl}oxy)({[({bis[(2-ethylhexyl)oxy]phosphoryl}oxy)(hydroxy)phosphoryl]oxy})[(2-hydroxyacetyl)oxy]titanio]oxy}({bis[(2-ethylhexyl)oxy]phosphoryl}oxy)phosphinic acid

Sampling and analysis

Analytical monitoring:

yes

Remarks:

LC-MS/MS

Details on sampling:

- Loading rates: control, 1, 10, 100 mg/L
- Sampling method: Samples were collected from fresh parent test solution samples at 0 h (initiation) and from a composite of spent replicate solutions at 48 h (termination) of the test. At each sampling point, a 5 mL sample was collected from the control and each test substance treatment.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The 1.00, 10.0, and 100 mg/L test item solutions were prepared as water accommodated fractions (WAF) by weighing 0.00200, 0.0200, and 0.200 g, of the substance respectively, into glass carboys containing 2 L of dilution water (freshwater). Solutions were covered with Parafilm® sealing film and stirred on an electric stir plate for approx. 22 h at room temperature, with the stirring adjusted to provide a vortex ≤25% of the solution depth. The solutions were then allowed to settle for approx. 4 h.The test treatment collections were conducted by drawing the appropriate amount of test solutions through a glass siphon tube held approximately one inch from the bottom of each glass carboy. Prior to dispensing test treatment collections, approximately 100 mL of each test solution was drained through the syphon tube and discarded.
- Differential loading: yes
- Controls: The control solution contained dilution water without test item.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Age of parental stock (mean and range, SD): < 24 h
- Feeding during test: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

150 mg CaCO3/L

Test temperature:

20 ± 1°C

pH:

7.7 to 8.6

Dissolved oxygen:

7.6 to 8.5 mg/L (90 to 98% saturation)

Conductivity:

349 μS/cm

Nominal and measured concentrations:

Nominal Loading Rates: 0 (control), 1, 10, and 100 mg/L
Geom. Mean Measured concentrations:

Details on test conditions:

TEST SYSTEM
- Test vessel: 250-mL glass jars
- Fill volume: 200 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Blended freshwater
- Alkalinity: 170 mg CaCO3/L
- Culture medium different from test medium: not specified

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/ 8 h dark (30 min transition period)
- Light intensity: 584 to 688 lux

EFFECT PARAMETERS MEASURED: Mobility and sublethal effects at 24 and 48 h.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

WAF

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOELR

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

WAF

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: no
- Mortality of control: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The control and all test solutions appeared clear and colorless with no visible particulates, surface film, or precipitate throughout the test.
- Effect concentrations exceeding solubility of substance in test medium: no

Any other information on results incl. tables

BIOLOGICAL RESULTS

After 48 h of exposure, there were no mortalities in the control replicates and there were no mortalities observed in any test treatment replicates up to the highest nominal concentration of 100 mg/L. Neither were sublethal effects observed at any dose during the duration of the test exposures.

ANALYTICAL RESULTS

Table 1: Measured Concentrations of Diethylhexyl Phosphite, an Analytical Marker of the UVCB substance, during the 48-h Acute Toxicity Test with Daphnia magna.

Nominal Loading Rate Concentratiomn [mg/L]	Measured Concentration as mg/L
	0 h	48 h	Geometric Mean Measured Concentration*
control	<MQL	<MQL	<MQL
1	0.0015	<MQL	0.00067
10	0.00447	<MQL	0.00116
100	0.306	0.0393	0.11

* For calculations, ½ MQL (0.000300 mg/L) used where the measured concentration was <MQL.

VALIDITY CRITERIA

Table 2: Validity criteria for OECD 202.

Criterion from the guideline	Outcome	Validity criterion fulfilled
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized.	0%	yes
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels.	7.6 to 8.5 mg/L throughout the test	yes

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

For further details please refer to “Any other information on results incl. tables”.