4-trans-Butyl-4'-trans-propyl-[1,1'-bicyclohexyl]

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 05 - Jun 17, 2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: This study was performed according to GLP and the methods applied are fully compliant with OECD TG 203.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-trans-Butyl-4'-trans-propyl-[1,1'-bicyclohexyl]

EC Number:

619-232-4

Cas Number:

96624-52-1

Molecular formula:

C36H36

IUPAC Name:

4-trans-Butyl-4'-trans-propyl-[1,1'-bicyclohexyl]

Test material form:

other: solid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

The test medium (reconstituted water and test material) was prepared freshly. Therefore, the calibrated flask with test medium was treated in an ultrasonic device for 1 hour. Subsequently, the preparation was stirred with a magnetic stirrer for further 23 hours. Afterwards, the formulation was passed through a membrane filter with a pore size of 0.2 µm. The filtrate was used for the study.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebrafish
- Strain: Dania rerio
- Source: in house
- Length at study initiation: 2.0 ± 1.0 cm
- Weight at study initiation: 8.8 g (test material group), 6.5 g (control group)
- Method of breeding: standard
- Feeding during test: tetra min diet during acclimatization, with daphnia or artemia, or frozen mosquito larva daily


ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test or not): same
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

no

Test conditions

Hardness:

250 mg/L CaCO3

Test temperature:

23 -25 °C

pH:

7-8

Dissolved oxygen:

> 60%

Nominal and measured concentrations:

100 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 L
- Type (delete if not applicable): open
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per vehicle control (replicates): 1

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 12/12 hours

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

Clinical Examination: daily
loss of equilibrium, changes in swimming behavior, respiratory function, pigmentation, dead


TEST CONCENTRATIONS
- Spacing factor for test concentrations: -
- Test concentrations: 100 mg/l
- Results used to determine the conditions for the definitive study: pretest

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: no
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

No aquatic toxicity was observed in this test system up to a nominal concentration of 100 mg/L test material. The 96h LC50 and the 96h NOEC were above the water solubility limit and larger than 100 mg/L (nominal).