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Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April 1985
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
Study meets generally accepted scientific principles
Qualifier:
no guideline followed
Principles of method if other than guideline:
Static bioassay: method not specified
GLP compliance:
no
Specific details on test material used for the study:
Common name: TCPP
Product name: Antiblaze 80 (AB-80)
Purity: >95%
Analytical monitoring:
yes
Details on sampling:
Samples were taken at study start (0 h) and at the end of the study (96 h). Samples were acidified to pH 2 with 6N HCL to deminish mircobial degradation and refrigerated (4 °C).
Vehicle:
no
Test organisms (species):
Pimephales promelas
Details on test organisms:
Species: Obtained from commercial domestic hatcheries.
Test fish: Acclimated for more than 14 days in MTC well water before testing.
The fish length: 2.3 cm
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Remarks on exposure duration:
additionally: study duration was extanded to 168 hr.
Post exposure observation period:
The duration of the study was extended to 168 h in order to determine whether additional mortality would result fromth eextended exposure period.
Hardness:
Total hardness ( CaCO3 ) 262 mg/L
Test temperature:
22°C
pH:
7.97 - 8.37
Dissolved oxygen:
7.4 - 8.75 mg/L
no aeration during test
Nominal and measured concentrations:
Nominal conc.: 0.9, 4.9, 9.8, 49 and 98 mg/L .
measured 0 h: 0.644, 4.01, 4,95, 12.4, 23.5 and 10.2 mg/L
measured 96 h: 0.746, 4.58, 8.82, 30.1, 57.7 and 31.7 mg/L
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
51 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
9.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
behaviour
Duration:
120 h
Dose descriptor:
LC50
Effect conc.:
98 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
168 h
Dose descriptor:
LC50
Effect conc.:
74 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95%CL: 45 -98mg/l
Sublethal observations / clinical signs:

Concentration of test substance in water collected at t = 0 hrs. and t = 96 hrs. in all test chambers in relation to the nominal concentration

 Container  Nominal conc. in mg/L  Meassured conc. t = 0 h in mg/L   Recovery rate in %  Meassured conc. t = 96 h in mg/L     Recovery rate in %
1A   0  Control      
2A  0.9  0.644 71.6   0.746  82.9
3A  4.9  4.01 81.8   4.58  93.5
4A   9.8  4.95 50.5  8.82  90.0
5A  49  12.4 25.3   30.1  61.4
6A  98  23.5 24.0   57.7  58.9
6B  98  10.2 10.4   31.7  32.4

LC50s were calculated (using the binomial method) from measured concentrations at 0h and 96h.

Analytical monitoring was performed, and the result relate to measured concentrations as the recovery rate was in the range between 32% and 93%. The large variation in the recovery rates may be based on incomplete mixing of the test substance in the test vessels at test start. The test substance was direct weighed into the test vessels and vigorously stirred afterwards with a glass rod. At test end the recovery rates increase.

Validity criteria fulfilled:
yes
Remarks:
mortality in control <10%
Conclusions:
TCPP has an acute toxicty toward fish (Pimephales promelas) of 96h-LC50 of 51 mg/L (measured).
Executive summary:

The study is no guideline study but is well documented and meets generally accepted scientific prinicples. The toxicity study towards (Pimephales promelas) was conducted under static condictions. TCPP was directly applied to the test water. An analytical monitoring with GC-MS was performed bt leaded to small recoveries. A sthe recoveries increase with incubation time, it is assumed that TCPP was not dipersed probably at the beginning of the test (0 h). As TCPP is hydrolytically stable a abiotic degradation can be excluded.

The water quality is documented during the test duration. Mortality <10% is fulfilled as validity criterion.

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
March to May 1991
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
GLP
Qualifier:
according to guideline
Guideline:
other: UBA-Verfahrensvorschlag: "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, LC0, LC50, LC100; 48-96 h" (Mai 1984) Guideline proposal of the German Federal Environmental Agency (UBA)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
Daily review of the test concentrations as well as the control
Vehicle:
no
Details on test solutions:
TCPP was directly weighed
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrabaerbling (Brachydanio rerio)
- Source: HAMILTON BUCHANAN (Bad Lauterberg)
- ventilated test system
- loading rate: 10 animals/vessel (3 month old)
- Length: 2.5 - 3.5 cm
- Weight of the 10 fishes: 2.24 - 3.02 g
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
13.2 °dH
Test temperature:
20.9 - 21.8°C
pH:
7.5 - 7.9
Dissolved oxygen:
8.2 - 9.1 mg/L
Nominal and measured concentrations:
Nominal conc.: 10, 31.6, 100 and 316 mg/L
Measured conc.: 9.4, 28.4, 90.7 and 286 mg/l at 0 hours and 9.4, 29.4 mg/l at 96 hours
Details on test conditions:
- The test was conducted in an 5 l aquarium (300x135x200 mm) filled with the test medium (synthetic origin, prepared according to ISO)
- Application method: Test substance direct weight.
- The substance was direct weighed in water and treated 60 seconds at 8000 rpm with the Ultra-Turrax Stirrer.




Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
31.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
56.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Sublethal observations / clinical signs:

Table - Measured Test Concentrations






















































Nominal Concentration (mg/L)


0 h Measured Concentrtaion (mg/L)24 h Measured Concentrtaion (mg/L)48 h Measured Concentrtaion (mg/L)72 h Measured Concentrtaion (mg/L)96 h Measured Concentrtaion (mg/L)
0<LOQ<LOQ<LOQ<LOQ<LOQ
109.29.69.49.39.4
31.628.432.129.528.829.4
10090.7----
316286----

 


Table - Fish Mortality






















































Nominal Concentration (mg/L)


% Mortality (0h)% Mortality (24h)% Mortality (48h)% Mortality (72h)% Mortality (96h)
000000
1000000
31.600000
1000100---
3160100---
Validity criteria fulfilled:
not specified
Conclusions:
In a test with TCPP to fish (Danio rerio) a LC50 of 56.2 mg/L after 96 hours is obtained.
Executive summary:

The study was conducted according UBA-Verfahrensvorschlag: "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, LC0, LC50, LC100; 48-96 h" (Mai 1984) (Bayer AG, 1991) without deviations. An analytical monitoring with gas chromatography was performed. The values obtained are based on nominal concentrations as TCPP is stable in media within the incubation time and shows high recoveries. LC0 and LC100 were detected directly. LC50 value is calculated.

Description of key information

OECD 203 (eqiv.)(Meeks, 1985): 96h LC50 = 51 mg/L (meas.)
OECD 203 (equiv.)(Bayer AG, 1991): 96h LC50 = 56.2 mg/L (meas.)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
LC50
Remarks:
Based on lowest LC50 from two key studies.
Effect concentration:
51 mg/L

Additional information

There are several tests on toxicity to fish towards different species available. The most sensitive species is Pimephales promelas, resulting in the lowest effect concentration of 51 mg/l. Analytical monitoring was performed, and the result relates to measured concentrations as the recovery rate was in the range between 32% and 93%. The increasing recovery rate over test duration may be based on incomplete mixing of TCPP in the test vessels at test start. The test substance was directly weighed into the test vessels and vigorously stirred afterwards with a glass rod. At test end the recovery rates shows that the test substance was completely mixed. The results on analytical monitoring obtained in the toxicity test towards Danio rerio show stability of TCPP during the test. TCPP was directly weighed into the test vessels, but treated with an ultra turrax for 60 seconds. The recovery rates at the beginning of the test ranged from 91 % to 94 % showing already complete mixing at test start. The result obtained for Pimephales promelas is considered to be reliable, as it is in good agreement with the result determined for Danio rerio.