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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Acute Toxicities of Organic Chemicals to Fathead Minnows (Pimephales promelas)
Author:
Geiger DL, Brooke LT, Call DJ
Year:
1986
Bibliographic source:
Vol. III. Center for Lake Superior Environmental Studies, University of Wisconsin-Superior.
Reference Type:
publication
Title:
Unnamed
Year:
2015
Report Date:
2012

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: US-EPA: Acute Toxicities of Organic Chemicals to Fathead Minnows
Deviations:
not specified
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Specific details on test material used for the study:
- Chemical source: Aldrich Chernical Co.
- Purity: 97 %

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Sampling schedule: 0, 24, 48, 72 and 96 hours

Test solutions

Vehicle:
no
Details on test solutions:
Toxicant stock: 125 mg/L; Diluted (Stirred)

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
Cultured at U.S. EPA Environmental Research Laboratory-Duluth and the University of Wisconsin-Superior campus.

- Age: 28 days
- Mean length (mm): 21.8
- SD Length (mm): 1.293
- Mean weight (g): 0.151
- SD Weight (g): 0.0232
- Loading (g/L): 2.517

Holding Conditions:
- Temperature: 25 °C in flowing water
- Lightning: 16-hr light-controlled photoperiod
- Feeding: frozen adult brine shrimp (Artemia sp.)

Only groups of fish. having healthy appearance and no history of unusual thermal exposure or abnormally high mortality rate were used for toxicant
exposure. Test fish were not fed 24 hr before or during a test.

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
Lake Superior water: hardness 51.9 mg CaCO3/L; alkalinity 44.0 mg CaCO3/L
Test temperature:
24.1 °C
pH:
7.08
Dissolved oxygen:
6.8
Salinity:
n.a.
Conductivity:
No data
Nominal and measured concentrations:
Nominal test concentration: 0.49, 0.75, 1.2, 1.8 and 2.7 mg/L

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.76 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Confidence limit: 0.65-0.88 mg/L
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
0.69 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Confidence limit: 0.59-0.79 mg/L
Details on results:
Recovery: 100.5 %

Any other information on results incl. tables

Affected fish lost schooling behavior and swam near the tank surface. They were hyperactive and darkly colored, had increased respiration, and lost equilibrium prior to death. Tanks were changed daily to reduce bacterial growth and stabilize the concentrations.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
In the test with a,a,a-trifluoro-m-tolualdehyde (CAS no. 454-89-7) with fish (Pimephales promelas) a LC50 of 0.76 mg/L (confidence limit: 0.65-0.88 mg/L) after 96 hours is obtained. The study was conducted according to US-EPA: Acute Toxicities of Organic Chemicals to Fathead Minnows.
Executive summary:

The study was conducted according to US-EPA: Acute Toxicities of Organic Chemicals to Fathead Minnows. In the test with a,a,a-trifluoro-m-tolualdehyde (CAS no. 454-89-7) with fish (Pimephales promelas) a LC50 of 0.76 mg/L (confidence limit: 0.65-0.88 mg/L) after 96 hours is obtained.