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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Experimental phase: 16 Nov - 20 Nov 1998
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Version / remarks:
1992
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0, 50, 100 mg/L- Concentrations:
- Sample storage conditions before analysis: refrigerator
Vehicle:
no
Details on test solutions:
All test solutions were prepared separately for each concentration by diluting 0.5 and 1.0 g test substance in test water with an ultra-turrax stirrer.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: zebrafish
- Source: Zierfisch-Center Kloeckner, Ludwigshafen, Germany
- Age at study initiation: ca. 3 months (hatching date: 10 Aug 1998)
- Length at study initiation: 3.6 cm mean
- Weight at study initiation: 0.33 g mean

ACCLIMATION
- Acclimation period: ca. 1 month
- Type and amount of food during acclimation: TetraMin and artemia ad lib.
- Feeding frequency during acclimation: workdays
- Health during acclimation (any mortality observed): 0% mortality within 7 d prior to test

FEEDING DURING TEST : no
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
250 mg/L (CaCO3 in dilution water)
Test temperature:
22°C
pH:
8.3 - 8.4
Dissolved oxygen:
8.4 - 8.7 mg/L
Salinity:
N/A
Conductivity:
N/A
Nominal and measured concentrations:
Nominal: 0 (control), 50, 100 mg/L
Measured (arithmetic mean 1 h, 96 h): <1 (control), 49.1, 98.6 mg/L
Recovery: >97% in both concentrations
Details on test conditions:
TEST SYSTEM
- Test vessel: aquaria
- Type: open
- Material, size, headspace, fill volume: glass, 30 x 22 x 24 cm, 10 L
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.3 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: municipal water supply
- Chlorine: chlorine-free
- Ca/Mg ratio: 9:1
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 h

EFFECT PARAMETERS MEASURED: mortality and clinical symptoms at 1, 24, 48, 72 and 96 h
Reference substance (positive control):
not required
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
No mortality or any clinical symptoms observed in control, 50 and 100 mg/L
Results with reference substance (positive control):
N/A
Reported statistics and error estimates:
N/A
Validity criteria fulfilled:
yes
Conclusions:
The test item did not cause any mortality or clinical symptoms in fish up to 100 mg/L (nominal, analytically verified). The 96-h LC50 to Danio rerio is >100 mg/L.

Description of key information

The test item did not cause any mortality or clinical symptoms in fish up to 100 mg/L (nominal, analytically verified). The 96-h LC50 to Danio rerio is >100 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

The substance is not acutely harmful to fish.