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EC number: 701-054-4 | CAS number: -
Acute toxicity of Test Item was assessed with Acute Immobilisation Test on Daphnia magna, over an exposure period of 48 hours in a semi-static system.
Because no toxic effect was observed at the solubility level of the Test Item in the preliminary test, only this test concentration 100 mg/L Test Item nominal loading rate (WAF) at the solubility level of the Test Item in the test medium and one control group were tested in a limit test in the definitive study.
Based on the agreement with the Sponsor and due to the fact that the Test Item is an UVCB the analytical measurement was performed by TOC analysis. Only the sum of the carbon content of the Test Item could be measured this way.
Twenty animals, divided into 4 groups (glass beaker) of 5 animals each were used at the test and for the control group. All validity criteria were met during this study.
Under the conditions of this Daphnia magna acute immobilisation study the calculated and observed endpoints for the effect of Test Item were the followings:
The 24h, 48h, ELR50value: >100 mg/L nominal loading rate WAF
The 48h ELR100value: >100 mg/L nominal loading rate WAF
The 48h NOELR: 100 mg/L nominal loading rate WAF
The 48h LOELR: >100 mg/L nominal loading rate WAF
Based on the results of this study, the Test Item had no toxic effect at saturation on Daphnia; the ELR50results and the LOELR are higher than the solubility level of the Test Item in the test medium.
 Effective Loading rate.
 No-Observed Effect Loading rate.
 Lowest Observed Effect Loading rate.
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