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EC number: 204-599-1 | CAS number: 123-08-0
The objective of this study was to determine the effect of the test item on the immobilization of Daphnia magna.
Juvenile daphnids were exposed to different test item concentrations of nominal 0.0, 10.0, 17.8, 31.6, 56.3 and 100.0 mg/L in an open static system. 20 daphnids, divided into four replicates, each of five animals were used per test item group and the control group. The daphnids were observed for immobilization after 24 and 48 hours of exposure.
Samples of the test and control medium were taken directly after preparation and at the end of exposure (48h) and stored at ≤ -20°C until further analysis of the test item concentration via HPLC with UV detection.
The measured concentrations were between 93.0% and 100.1% of the nominal concentrations.
As the recoveries of the test item in the aqueous solutions were >80% of the nominal concentrations during the study, the EC50 values were calculated with the nominal concentrations.
No effect on the mobility of the daphnia was observed at the two lowest concentrations of nominal 10.0 and 17.8 mg/L. A concentration dependent immobilization was observed between 31.6 and 100.0 mg/L as given in the following table.
For the test item the following nominal EC50 values and 95% confidence intervals were determined:
24h EC50 74.1 mg/L (57.5 - 110.9 mg/L)
48h EC50 41.1 mg/L (34.7 - 48.7 mg/L)
Under the conditions of the present study, the test item showed a 48h EC50 of 41.1 mg/L.
This study was performed according to GLP and the methods applied are fully compliant with OECD TG 202. Under the conditions of the present study, the test item showed a 48h EC50 of 41.1 mg/L.
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