Sodium 3-(2-propyn-1-yloxy)-1-propanesulfonate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017-09-20 - 2017-10-19 (experimental phase)

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

OECD Guidelines for the Testing of Chemicals Part 301 F, adopted 17. Jul. 1992 „Manometric Respirometry Test“

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Version / remarks:

Council Regulation (EC) No. 440/2008, Guideline C.4-D, adopted 30. May 2008 “Manometric Respirometry Test”

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Sodium 3-(2-propyn-1-yloxy)-1-propanesulfonate

EC Number:

608-454-7

Cas Number:

30290-53-0

Molecular formula:

C6H9O4NaS

IUPAC Name:

Sodium 3-(2-propyn-1-yloxy)-1-propanesulfonate

Test material form:

liquid

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Sponsor

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test item was stored in a closed vessel at room temperature 16.0 – 21.5 °C, away from light and humidity.

OTHER SPECIFICS: Molecular formula: C2.81H5.35Na0.49O2.48S0.49

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from a biologic sewage treatment plant was used. The chosen plant is treating mostly household sewage.
The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf.
Date of collection: 21. Sep. 2017, batch no: 20170921.
- Pretreatment: The sludge was filtrated, washed with tap water 2x, then washed with and re-suspended in test medium. It was then aerated.
- Concentration of sludge: The dry matter was determined as 4980 mg suspended solids/L.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

88 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

99.81 mg/L

Based on:

ThOD

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium:
Stock Solutions
Solution a
Potassium dihydrogen phosphate (KH2PO4) 8.5 g
Dipotassium hydrogen phosphate (K2HPO4) 21.75 g
Disodium hydrogenphosphate dihydrate (Na2HPO4*2H2O) 33.4 g
Ammonium chloride (NH4Cl) 0.5 g
H2O demin. ad 1000 mL
The pH was to 7.4.
Solution b
Calcium chloride (CaCl2) 27.5 g
H2O demin. ad 1000 mL
Solution c
Magnesium sulphate heptahydrate (MgSO4*7H2O) 22.5 g
H2O demin. ad 1000 mL
Solution d
Iron(III) chloride hexahydrate (FeCl3*6H2O) 0.25 g
Disodium ethylene-diaminetetraacetate dihydrate (Na2EDTA*2H2O) 0.4 g
H2O demin. ad 1000 mL
Test Medium
The medium was freshly prepared (volumes were adapted to final volume needed in the test).
Composition:
Solution a 10 mL
Solution b 1 mL
Solution c 1 mL
Solution d 1 mL
H2O demin ad 1000 mL
Mercury Chloride
HgCl2 (10 g/L), used for poisoning of abiotic flasks
Soda lime
CO2 adsorbent

- Test temperature: 20.9 - 21.2 °C
- pH: 7.2 - 7.4

TEST SYSTEM
- Culturing apparatus: 500 mL-flasks were used as test vessels (590 mL total volume including measuring head).
All glassware was cleaned with the laboratory cleaning agent and then rinsed with tap water (3x), diluted HCl (1x), tap water (3x) and deionised water (3x).
Volume test solution was 250 mL (toxicity flask 125 mL)
- Number of culture flasks/concentration:
Number of control flasks: 3 (medium and inoculum)
Number of positive control flasks: 3 (sodium-benzoate, medium and inoculum)
Number of test vessels: 3 (test item, medium and inoculum)
Abiotic control: 1 (test item and 10 mL 1% HgCl2 solution/L)
Toxicity control: 1 (200 mg/L ThOD, 100 mg/L ThOD each of test item and positive control)

- Details of trap for CO2 and volatile organics if used: soda lime
- Other: Concentration of suspended solids: 24.9 mg/L, 24.7 mg/L in the toxicity control

CONTROL AND BLANK SYSTEM
see above

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

Test performance:

All validity criteria were met.

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

Remarks on result:

other: not readily biodegradable

Remarks:

degradation missed 60 % in the course of the test

BOD5 / COD results

Results with reference substance:

Degradation of positive control was > 60% after 3 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The study was performed according to OECD TG301F and EU Method C.4-D (GLP) with no deviation and is therefore considered to be of the highest quality (reliability Klimisch 1). The validity criteria of the test system are fulfilled. No degradation of the test item and therefore no 10-day–window could be detected. No observations were made which might cause doubts concerning the validity of the study outcome. The result of the test can be considered valid.

Executive summary:

The biodegradability of the test substance was investigated according to EU method C.4 -D and OECD guideline 301 F (GLP).

The test item Propargyl 3-sulfopropyl ether, sodium salt (POPS-Na) was tested using a concentration of nominal 88 mg test item/L (equivalent to a theoretical oxygen demand of 100 mg O2/L). Activated sludge was used as inoculum. The test was left running for 28 days.

All validity criteria were met. The positive control reached the pass level of 60% on day 3 (criterion:£14).

The following data could be determined for the test item Propargyl 3-sulfopropyl ether, sodium salt (POPS-Na):

degradation at the end of the test: 0 %

As degradation missed 60% in the course of the test, Propargyl 3-sulfopropyl ether, sodium salt (POPS-Na) is considered as not readily biodegradable following OECD 301F/EU C.4-D.