Trehalose

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 1997 - August 1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

Principles of method if other than guideline:

The test was performed in accordance with Notice “Test methods for new chemical substances –
Biodegradability test of chemical substances by microorganisms, etc.” specified in “Kan-Ho-gyo
No. 5”, “Yaku-Hatsu No. 615” and “49Ki-Kyoku No. 392” issued in1972.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Trehalose

EC Number:

202-739-6

EC Name:

Trehalose

Cas Number:

99-20-7

Molecular formula:

C12H22O11

IUPAC Name:

trehalose

Test material form:

solid: crystalline

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 609111
- Expiration date of the lot/batch: not stated
- Purity test date: not stated

RADIOLABELLING INFORMATION (if applicable)
not applicable

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: not stated
- Stability under test conditions: assumed stable
- Solubility and stability of the test substance in the solvent/vehicle: assumed stable
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: assumed stable

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
not specified

FORM AS APPLIED IN THE TEST (if different from that of starting material)

TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)

OTHER SPECIFICS:

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

Standard activated sludge purchased from Chemicals Inspection &
Testing Institute, Japan (now Chemicals Evaluation and Research
Institute, Japan), Chemicals Safety Center on July 24, 1997 was used
for the test.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation 1

Parameter followed for biodegradation estimation:

DOC removal

Parameter followed for biodegradation estimation 2

Parameter followed for biodegradation estimation:

O2 consumption

Parameter followed for biodegradation estimation 3

Parameter followed for biodegradation estimation:

test mat. analysis

Details on study design:

TEST CONDITIONS
- Composition of medium: ml of A, B, C and D solutions specified in JIS K
0102, 21(1) was added to 1976 mL of deionized water
- Additional substrate: none
- Solubilising agent (type and concentration if used): none
- Test temperature: 25 +- 1 °C
- pH: 7
- pH adjusted: yes
- CEC (meq/100 g): not stated
- Aeration of dilution water: not stated
- Suspended solids concentration: not stated
- Continuous darkness: not stated
- Other: not stated

TEST SYSTEM
- Culturing apparatus: not stated
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: not stated
- Method used to create anaerobic conditions: not applicable
- Measuring equipment: no further equipment during test
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: no
- Details of trap for CO2 and volatile organics if used: not applicable
- Other:

SAMPLING
- Sampling frequency: weekly
- Sampling method: by device (Ohkura Electric Co., Ltd.
“Coulometer OM-3001A”)
- Sterility check if applicable: not specified
- Sample storage before analysis: not specified
- Other:

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: no
- Other:

STATISTICAL METHODS: none applied

Reference substance

Reference substance:

aniline

Results and discussion

Preliminary study:

none

Test performance:

No information reported on performance of test

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

>= 68 - <= 74

Sampling time:

28 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

>= 62 - <= 67

Sampling time:

21 d

% Degradation 3

Parameter:

% degradation (O2 consumption)

Value:

>= 57 - <= 60

Sampling time:

14 d

% Degradation 4

Parameter:

% degradation (O2 consumption)

Value:

>= 47 - <= 49

Sampling time:

7 d

% Degradation 5

Key result

Parameter:

% degradation (DOC removal)

Value:

>= 98 - <= 100

Sampling time:

28 d

% Degradation 6

Parameter:

% degradation (test mat. analysis)

Value:

100

Sampling time:

28 d

Details on results:

Direct measurement of Trehalose in blank control by HPLC showed 101% recovery at day 28.

Any other information on results incl. tables

 

BiodegradabilitycalculatedfromBOD

Culture Bottle No.	Items	Theoretical oxygen demand (mg)	Day 7		Day 14		Day 21		Day 21
			Measured value (mg)	Degradability (%)	Measured value (mg)	Degradability (%)	Measured value (mg)	Degradability (%)	Measured value (mg)	Degradability (%)
1	Test article section 1	33.7	19.6	47	24.3	57	26.7	62	29.3	68
2	Test article section 2	33.7	19.9	48	24.6	58	28.5	67	32.5	78
3	Test article section 3	33.7	20.4	49	25.1	60	28.3	67	31.2	74
4	Aniline section	90.8	53.3	55	70.3	72	72.3	73	72.8	73
5	Basicbreathing section	-	3.8	-	5.0	-	5.8	-	6.3	-
6	Stability section	-	0.0	-	0.0	-	0.0	-	0.0	-

 

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable, but failing 10-day window

Conclusions:

As the results calculated from BOD, the test article degradability was 68%, 78% and 74%
(average 73%).
As the results calculated from DOC, the test article degradability was 100%, 97% and 98%
(average 98%).
By HPLC method, the test article detectable at the end of test period,
the degradability of test article was all 100%.
Based on the above results, it was concluded that test article, trehalose, is ready biodegradable
under the conditions specified by Test methods for new chemical substances –
Biodegradability test of chemical substances by microorganisms, etc.” specified in “Kan-Ho-gyo
No. 5”, “Yaku-Hatsu No. 615” and “49Ki-Kyoku No. 392” issued in1972.