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EC number: 826-490-3 | CAS number: 1971906-58-7
Immobilization effect of test item toDaphnia magna.
Nominal test item
No. of immobilizedDaphnia
% of immobilizedDaphnia
0.0 (negative control)
0.0 (solvent control)
Title: YELLOW 2747: Acute toxicity to Daphnia magna in a 48-hour immobilization test under static exposure.
Evaluations: Immobilization after 24 and 48 hours of exposure, abnormal behaviour or appearance were compared with corresponding parameters in the negative control.
Test concentrations: Negative control (reconstituted water without test item), solvent control (reconstituted water without test item but with the same concentration of solvent as in test item concentration tested) and 0.043, 0.094, 0.207, 0.455 and 1.0 mg test item/L.
Analytical check of tested concentrations: not performed
Exposure regime: static
Test duration: 48 hours
Test environment: controlled environment room.
Monitoring of test medium quality: pH and oxygen concentration was measured at the start and at the end of the test, in the negative control, solvent control and test item solution.
pH of test medium: 7.43 – 7.57
Oxygen Concentration: 6.10 – 6.64 mg O2/L
Light intensity: 1356 – 1500 Lux
Light Regime: 16 hours light and 8 hours dark
Test temperature: 20.0°C
Results expression: The biological results were referred to nominal concentration of test item.
Results, calculated in terms of nominal test item concentration, were as follows:
Time (h) EC0(mg/L) EC100(mg/L) EC50(mg/L) NOEC(mg/L)
24 1.0 > 1.0 > 1.0 1.0
48 0.043 > 1.0 0.6 0.094
LCL, Lower Confidence Level and UCL, Upper Confidence Level= 0.361 – 1.575
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