Hydrogen [4-[[4-(diethylamino)phenyl][4-[ethyl[(3-sulphonatobenzyl)amino]-o-tolyl]methylene]-3-methylcyclohexa-2,5-dien-1-ylidene](ethyl)(3-sulphonatobenzyl)ammonium, sodium salt

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: UBA-Verfahrensvorschlag: "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, LC0, LC50, LC100; 48-96 h" (Mai 1984)

Version / remarks:

Guideline proposal of the German Federal Environmental Agency (UBA)

GLP compliance:

no

Specific details on test material used for the study:

Batch no. 866065

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Source: West Aquarium GmbH (Germany)
Temperature: 20 - 24 °C
Dissolved oxygen: > 5 mg/L
Mortalities during acclimatisation period: < 5 %
Medication: none
Keeping water: dechlorinated Tap water, pH: 6.3 – 6.8, oxygen: >= 90 % of saturation

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

15 °dH

Test temperature:

21.4 - 24.1 °C

pH:

6.5 - 7.1

Dissolved oxygen:

7.8 - 8.9 O2 mg/L

Nominal and measured concentrations:

1 mg/L, 10 mg/L, 100 mg/L nominal concentration of test item

Details on test conditions:

Test vessels: glass aquaria holding 5 L of test media covered with glass plates
Experimental design: 3 test concentrations, 10 animals per test concentration, no feeding during the exposure period
Method of initiation: animals were placed in prepared media
Photoperiod: 16 h light: 8 h dark
Test item concentration/s: 1, 10 and 100 mg/L (nominal concentration)
Medium renewal: none
Criteria of effects: The criterion of death used in this study was the absence of response to physical stimulation every 24 h (observations on mortality at 0, 24, 48, 72 and 96 hours)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

number of fish: normal behaviour/ abnormal behaviour/ dead

test item concentration	1 mg/L	10 mg/L	100 mg/L
0 h	10/0/0	10/0/0	10/0/0
24 h	10/0/0	10/0/0	10/0/0
48 h	10/0/0	10/0/0	10/0/0
72 h	10/0/0	10/0/0	10/0/0
96 h	10/0/0	10/0/0	10/0/0

Conclusions:

In a study with fish (Danio rerio) a LC50 of more than 100 mg/L after 96 hours is obtained.

Executive summary:

The study was conducted according to Umweltbundesamt (UBA) -Verfahrensvorschlag: "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, Mai 1984" with fish (Danio rerio). The fish were exposed to 3 nominal concentrations of Special Brilliant Blue FFR (1, 10 and 100 mg/L). 10 fish were tested per concentration level. A LC50 of more than 100 mg/L after 96 hours is obtained.

Description of key information

The study was conducted according to Umweltbundesamt (UBA) -Verfahrensvorschlag: "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, Mai 1984" with fish (Danio rerio). The fish were exposed to 3 nominal concentrations of Special Brilliant Blue FFR (1, 10 and 100 mg/L). 10 fish were tested per concentration level. A LC50 of more than 100 mg/L after 96 hours is obtained.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Should read LC50 > 100 mg/L