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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Qualifier:
according to
Guideline:
other: UBA-Verfahrensvorschlag: "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, LC0, LC50, LC100; 48-96 h" (Mai 1984)
Version / remarks:
Guideline proposal of the German Federal Environmental Agency (UBA)
GLP compliance:
no
Specific details on test material used for the study:
Batch no. 866065
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Source: West Aquarium GmbH (Germany)
Temperature: 20 - 24 °C
Dissolved oxygen: > 5 mg/L
Mortalities during acclimatisation period: < 5 %
Medication: none
Keeping water: dechlorinated Tap water, pH: 6.3 – 6.8, oxygen: >= 90 % of saturation
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
15 °dH
Test temperature:
21.4 - 24.1 °C
pH:
6.5 - 7.1
Dissolved oxygen:
7.8 - 8.9 O2 mg/L
Nominal and measured concentrations:
1 mg/L, 10 mg/L, 100 mg/L nominal concentration of test item
Details on test conditions:
Test vessels: glass aquaria holding 5 L of test media covered with glass plates
Experimental design: 3 test concentrations, 10 animals per test concentration, no feeding during the exposure period
Method of initiation: animals were placed in prepared media
Photoperiod: 16 h light: 8 h dark
Test item concentration/s: 1, 10 and 100 mg/L (nominal concentration)
Medium renewal: none
Criteria of effects: The criterion of death used in this study was the absence of response to physical stimulation every 24 h (observations on mortality at 0, 24, 48, 72 and 96 hours)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

number of fish: normal behaviour/ abnormal behaviour/ dead

 test item concentration  1 mg/L  10 mg/L  100 mg/L
 0 h  10/0/0  10/0/0  10/0/0
 24 h  10/0/0

 10/0/0

 10/0/0

 48 h

 10/0/0

 10/0/0

 10/0/0

 72 h

 10/0/0

 10/0/0

 10/0/0

 96 h

 10/0/0

 10/0/0

 10/0/0

Conclusions:
In a study with fish (Danio rerio) a LC50 of more than 100 mg/L after 96 hours is obtained.
Executive summary:

The study was conducted according to Umweltbundesamt (UBA) -Verfahrensvorschlag: "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, Mai 1984" with fish (Danio rerio). The fish were exposed to 3 nominal concentrations of Special Brilliant Blue FFR (1, 10 and 100 mg/L). 10 fish were tested per concentration level. A LC50 of more than 100 mg/L after 96 hours is obtained.

Description of key information

The study was conducted according to Umweltbundesamt (UBA) -Verfahrensvorschlag: "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, Mai 1984" with fish (Danio rerio). The fish were exposed to 3 nominal concentrations of Special Brilliant Blue FFR (1, 10 and 100 mg/L). 10 fish were tested per concentration level. A LC50 of more than 100 mg/L after 96 hours is obtained.

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

Should read LC50 > 100 mg/L