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Diss Factsheets
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EC number: 948-406-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- See principles of methods
- Principles of method if other than guideline:
- In view of the difficulties associated with the evaluation of aquatic toxicity of poorly water soluble test items, a modification of the standard method for the preparation of aqueous media was performed. (ECETOC, 1996 and OECD 2019). A series of solvent stock solutions was prepared in dimethylformamide in a series of nominal concentrations . An aliquot of each solvent stock solution was separately dispersed in test water with the aid of magnetic stirring fto give the desired nominal test concentrations.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken from the sovent control and each test group from the bulk test at the start, from additional media run alongside the test at 24 and 48 hours, and from the pooled replicates at 72 hours for inalysis. The samples were placed in vials containing 5 mL of Tetrahydrofuran and stored until analysis. Additional samples of each test concentration were incubated alongside the test to provide samples for analysis at 24 and 48 hours. Samples were either analysed on the day or frozen until analysis
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Range finder tests were carried out with and without solvent. The method preferred for the definitive test used Dimethyl formamide as the vehicle as the substance alone could not be suitably solubilised using a water accomodated fraction approach. Aliquots of stock solution were diluted in test medium to give required final test concentrations.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Dimethyl formamide (DMF)
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): 10g/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no. The concentration and stability of the test item in the test preparations were verified by chemical analysis at 0, 24, 48 and 72 hours - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: CCAP 278/4.
- Source (laboratory, culture collection): Culture Collection of Algae and Protozoa, SAMS Research Services Ltd, Scottish Marine Institute, Oban, Argyll, Scotland.
ACCLIMATION
- Acclimation period: 3 to 4 days before the start of the test, inoculum cultures of algae was set up at an initial cell density of approximately 103 cells/mL. The plugged flasks were agitated under illumination until the algal cell density was approximately 105 to 106 cells/mL.
- Culturing media and conditions (same as test or not): yes, except for the addition of 250 mg/L of sodium bicarbonate to prevent inhibition of growth due to the restriction in gaseous exchange associated with conducting the test in an enclosed system.
- Any deformed or abnormal cells observed: not reported - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- not reported
- Test temperature:
- 24 ±1 ºC
- pH:
- 7.6 at 0 hours increasing to pH 10.0 at 72 hours.
- Dissolved oxygen:
- not reported
- Salinity:
- not applicable
- Conductivity:
- not reported
- Nominal and measured concentrations:
- Nominal: 0.01, 0.026, 0.064, 0.16, 0.40mg/L. Concentrations declined during the study. Geometric means of the measured concentrations at 24, 48 and 72 hours were 0.0061, 0.0074, 0.035, 0.12, 0.25mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Conical flasks
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: Glass, 250mL completely filled
- Aeration: no
- Initial cells density: 5000 cells/mL
- Control end cells density: ~600000cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): 3
GROWTH MEDIUM
- Standard medium used: similar:
NaNO3 25.5 mg/L
MgCl2.6H2O 12.16 mg/L
CaCl2.2H2O 4.41 mg/L
MgSO4.7H2O 14.6 mg/L
K2HPO4 1.044 mg/L
NaHCO3 15.0 mg/L
H3BO3 0.186 mg/L
MnCl2.4H2O 0.415 mg/L
ZnCl2 0.00327 mg/L
FeCl3.6H2O 0.160 mg/L
CoCl2.6H2O 0.00143 mg/L
Na2MoO4.2H2O 0.00726 mg/L
CuCl2.2H2O 0.000012 mg/L
Na2EDTA.2H2O 0.30 mg/L
TEST MEDIUM / WATER PARAMETERS
- No data
OTHER TEST CONDITIONS
- Sterile test conditions: sterile conditions not specifically mentioned
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 7000lux warm white light 380-730nm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Counter multisizer particle counter, three determinations per sample.
TEST CONCENTRATIONS
- Range finding study: Two initial tests run using a water accomodated fraction approach with nominal loadings of 1, 10, 100mg/L. A second study was run at nominal loadings of 0.001, 0.01, 0.1 and 1.0mg/L. Due to inconsistenc of results using an initial WAFapproach and test concentrations of 0.04, 0.09, 0.2, 0.5 and 1.2mg/L, the approach was taken to use a DMFvehicle 'solvent spike' approach and a further range finder was run using nominal concentrations of 0.001, 0.1 and 1.0mg/L via a DMF solution. This proved acceptable so this approach was used for the definitive study. - Reference substance (positive control):
- yes
- Remarks:
- A positive control test using potassium dichromate as the reference item was performed twice in a 12 month period to demonstrate satisfactory conditions of the test. The last one performed (July 2019) was satisfactory.
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.052 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.035 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.007 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- No unusual observations reported that might invalidate the results - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- No Observed Effect Concentration based on growth rate: 0.25 mg/L
- No Observed Effect Concentration based on yield: 0.25 mg/L
- Lowest Observed Effect Concentration based on growth rate: 0.50 mg/L
- Lowest Observed Effect Concentration based on yield: 0.50 mg/L - Reported statistics and error estimates:
- Growth rate 95% confidence limit 0.043 - 0.062mg/L
Yield 95% confidence limit 0.013 - 0.018mg/L - Executive summary:
In a guideline OECD201 Freshwater algae growth inhibition test, the substance THI TM exhibited a 72 hr NOEC of 0.0074mg/L and an EC50 of 0.054mg/L based on growth rate. The substance was solubilised using DMF as a vehicle.
Reference
Description of key information
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.052 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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