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EC number: 700-242-3 | CAS number: 62037-80-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
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- Auto flammability
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- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Endpoint summary
- Stability
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Carcinogenicity
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- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data

Dermal absorption
Administrative data
- Endpoint:
- dermal absorption in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 428 (Skin Absorption: In Vitro Method)
- Deviations:
- no
- Remarks:
- The study was conducted according to the guideline in effect at the time of study conduct.
- GLP compliance:
- no
Test material
- Reference substance name:
- Reference substance 002
- Cas Number:
- 62037-80-3
- Details on test material:
- - Purity: 86%
Constituent 1
- Radiolabelling:
- no
Test animals
- Species:
- other: in vitro human and rat skin
Results and discussion
Percutaneous absorption
- Remarks on result:
- other: Based on the aqueous concentration of the applied test material (124 mg/mL) and steady-state penetration results, the Kp for human and rat skin was calculated to be 5.0E-5 ± 4.3E-5 cm/h and 5.7E-4 ± 4.3E-5 cm/h, respectively.
Any other information on results incl. tables
Following application of an infinite dose of an aqueous dilution of the test substance to dermatomed human and rat skin, the skin barrier properties had degraded by approximately 50% (independent of species) over the 24 hour contact time. Lag time was 1.73 ± 1.01 hours and 0.82 ± 0.77 hours for human and rat skin, respectively. Steady-state penetration was 6.2 ± 5.3 μg/cm²/h and 70 ± 5.3 μg/cm²/h for human and rat skin, respectively. Based on the aqueous concentration of the applied test material (124 mg/mL) and steady-state penetration results, the Kp for human and rat skin was calculated to be 5.0E-5 ± 4.3E-5 cm/h and 5.7E-4 ± 4.3E-5 cm/h, respectively. Based on the aqueous concentration of the applied test material (124 mg/mL) and steady-state penetration results, the Kp for human and rat skin was calculated to be 5.0E-5 ± 4.3E-5 cm/h and 5.7E-4 ± 4.3E-5 cm/h, respectively.
Applicant's summary and conclusion
- Conclusions:
- Based on the aqueous concentration of the applied test material (124 mg/mL) and steady-state penetration results, the Kp for human and rat skin was calculated to be 5.0E-5 ± 4.3E-5 cm/h and 5.7E-4 ± 4.3E-5 cm/h, respectively.
- Executive summary:
The permeability coefficient (Kp) was determined using human and rat skin mounted onto an in vitro static diffusion cell. The donor and receptor chambers were filled with saline and the water-jacketed cells maintained at 32ºC using a re-circulating water bath. Following a brief equilibration, membrane integrity was confirmed using electrical impedance (n=3 replicates per species). Saline was then removed from the donor chamber and the test material, a 86% aqueous solution, which had been further diluted with water to a concentration of 124 mg/mL, was applied to the epidermal surface via the donor chamber as an infinite dose (pilot experiments had suggested application of the neat test substance would likely degrade the barrier properties of the skin, so a more dilute sample was used). The donor chamber was then occluded with Parafilm® and serial receptor fluid samples (100 μL) were collected at 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 12 and 24 hours and analyzed for test substance anion by LC/MS/MRM (329>285 m/z). The cumulative amount of anion detected in the receptor fluid at each sampling time point was normalized to the exposure area (0.64 cm²) and the results plotted as the cumulative amount penetrated (μg/cm²) versus time (in hours) to produce a penetration profile. A permeability coefficient (Kp in cm/h) was calculated by dividing the penetration rate or slope of the line at steady-state (μg/cm²/h) by the concentration of the applied chemical (μg/cm³).
Following application of an infinite dose of an aqueous dilution of test substance to dermatomed human and rat skin, the skin barrier properties had degraded by approximately 50% (independent of species) over the 24 hour contact time. Lag time was 1.73 ± 1.01 hours and 0.82 ± 0.77 hours for human and rat skin, respectively. Steady-state penetration was 6.2 ± 5.3 μg/cm²/h and 70 ± 5.3 μg/cm²/h for human and rat skin, respectively.
Based on the aqueous concentration of the applied test material (124 mg/mL) and steady-state penetration results, the Kp for human and rat skin was calculated to be 5.0E-5 ± 4.3E-5 cm/h and 5.7E-4 ± 4.3E-5 cm/h, respectively.
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