4-allylanisole

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-allylanisole

EC Number:

205-427-8

EC Name:

4-allylanisole

Cas Number:

140-67-0

Molecular formula:

C10H12O

IUPAC Name:

1-methoxy-4-(prop-2-en-1-yl)benzene

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Activated sludge from a biologic sewage treatment plant was used. The chosen plant is treating mostly household sewage.
The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf.
Date of collection: 10. May 2017, batch no: 20170510.
The sludge was filtrated, washed with tap water 2x, then washed with and re-suspended in test medium. It was then aerated.
The dry matter was determined as 3800 mg suspended solids/L.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

37 mg/L

Based on:

ThOD

Remarks:

(corresponding to 100.4 mg/L ThOD)

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Key result

Parameter:

% degradation (O2 consumption)

Value:

46

Sampling time:

10 d

% Degradation 2

Key result

Parameter:

% degradation (O2 consumption)

Value:

48

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Oxygen consumption in the blank controls and in the positive controls was normal, indicating that the test system has worked properly.
The degree of biodegradation reached 48 % after 28 days. The 10-day-window began on day 6, at its end (day 16), 46 % were reached, staying below the pass level of 60% given in the OECD guideline. Therefore, when applying the 10-day-window, the test item Estragole is not readily biodegradable.