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EC number: 283-634-2 | CAS number: 84696-15-1 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Zingiber officinalis, Zingiberaceae.
Table8.3–a Calculated peptide depletion values for the Lys-Peptide (Assay 1)
Positive control Rep. 1
Positive control Rep. 2
Positive control Rep. 3
Test item Rep. 1
Test item Rep. 2
Test item Rep. 3
Table8.3–b Calculated peptide depletion values for the Lys-Peptide (Assay 2)
Table8.3–c Calculated peptide depletion values for the Cys-Peptide (Assay 1)
Table8.3–d Calculated peptide depletion values for the Cys-Peptide (Assay 2)
Mean depletion of both peptides after incubation with the test item: 27.81 % (Assay 1) and 28.10 % (Assay 2)
a) The mean peptide depletion value for the positive control cinnamaldehyde should be 60.8 % - 100 % with a maximum standard deviation (SD) of < 14.9 % for the Cys-peptide.
b) The mean peptide depletion value for the positive control 2,3-butanedione should be 10 % - 45 % with a maximum standard deviation < 11.6 % for the Lys-peptide.
c) The maximum standard deviation for the test item replicates should be < 14.9 % for the percent cysteine depletion and < 11.6 % for the percent lysine depletion
a) The mean peptide depletion and standard deviation of the three replicates of the positive control cinnamaldehyde were in the acceptable range of 60.8 – 100.0 % and ≤ 14.9 %, respectively, for the Cys-peptide.
b) The mean peptide depletion and standard deviation of the three replicates of the positive control 2,3-Butanedione were in the acceptable range of 10.0 – 45.0 % and ≤ 11.6 %, respectively, for the Lys-peptide.
c) The maximum standard deviation for the test item replicates was < 14.9 % for the percent cysteine depletion for the test item.
The maximum standard deviation for the test item replicates was < 11.6 % for the percent lysine depletion for the test item.
According to the test guideline, the reactivity is classified as “high”, “moderate”, “low” or “minimal” using the Cysteine 1:10/Lysine 1:50 prediction model shown in Table8.4–a.
Table8.4–a Evaluation of results according to the Cysteine 1:10/Lysine 1:50 prediction model
Mean peptide depletion[%]
0 – ≤ 6.38
> 6.38 – ≤ 22.62
> 22.62 – ≤ 42.47
> 42.47 - ≤ 100
The mean peptide depletion in the Lys-peptide and Cys-peptide assay was 27.81 %, therefore the test had to be repeated for verification of this result. The mean peptide depletion in the Lys-peptide and Cys-peptide in the repeated experiment was 28.10 %. The result of the first experiment was considered verified:
DPRA Prediction: Positive
Reactivity class: Moderate
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