Ginger, ext.

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

1

Reference substance name:

Ginger, ext.

EC Number:

283-634-2

EC Name:

Ginger, ext.

Cas Number:

84696-15-1

Molecular formula:

N/A

IUPAC Name:

Essential oil extract of Zingiber officinalis (Zingiberaceae) root GINGER ESSENTIAL OIL Ginger Oil Ginger Oleoresin Ginger, ext Ginger, ext. Ginger, Extrakt HE Zingiber officinalis INCI name: Zingiber Officinale (Ginger) Root Extract OILS, GINGER Reaction mass of camphene and pin-2(3)-ene and 7-methyl-3-methyleneocta-1,6-diene and pin-2(10)-ene and cineole and [S-(R*,S*)]-5-(1,5-dimethylhexen-4-yl)-2-methyl-1,3-cyclohexa-1,3-diene and (R)-p-mentha-1,8-diene Zingiber officinalis, Zingiberaceae.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

48 h

Remarks on exposure duration:

24 and 48 h

Post exposure observation period:

24h

Test conditions

Test temperature:

21 °C ± 1 °C,

pH:

7.8 ± 0.5

Dissolved oxygen:

Dissolved oxygen was > 7 mg/l (minimum acceptable value)

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

other: Cv

Remarks:

Cv%=|Tt48−Tt0|/Tt48 ×100 Tt48 is the titer determined at 48 hours, Tt 0 is the titer determined at 0 hours

Effect conc.:

ca. 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Remarks:

3.08%

Basis for effect:

mobility

Details on results:

At the end of the definitive test, EC50 was calculated using the EPA software PROBIT. 95% confidence limits was also calculated. The PROBIT elaboration was attached to the raw data.
EC50: 8.752 mg/l (confidence limits 7.022 mg/l – 10.709 mg/l)

Any other information on results incl. tables

Test Solution	%Immobilization (24 h)	%Immobilization (48 h)	Initial pH	Final pH	Initial Dissolved Oxygen	Final Dissolved Oxygen
C1 (500 mg/l)	80 %	100 %	7.83	7.52	8.89	9.34
C2 (250 mg/l)	50 %	90 %	7.82	7.43	8.56	8.90
C3 (125 mg/l)	30 %	70 %	7.83	7.36	9.24	9.95
C4 (62.5 mg/l)	10 %	30 %	7.92	7.29	9.60	9.52
C5 (31.3 mg/l)	0 %	0 %	7.99	7.23	9.17	9.72
Negative Control	0 %	0 %	8.03	7.62	8.78	9.11

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

the concentrations used in this test were: 3.2 mg/l – 1.8 mg/l – 1 mg/l – 0.56 mg/l – 0.32 mg/l. Results of the tests were reported in the control chart 775/GxP. The calculated EC50 resulted between 0.6 mg/l and 2.1 mg/l.

Conclusions:

According to OECD 202:2004, under the test conditions applied, the EC50 of the test substance is calculated as 8.752 mg/l.