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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report Date:
2018

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
saltwater
Limit test:
yes
Total exposure duration:
48 h
Remarks on exposure duration:
24 and 48 h
Post exposure observation period:
24h

Test conditions

Test temperature:
21 °C ± 1 °C,
pH:
7.8 ± 0.5
Dissolved oxygen:
Dissolved oxygen was > 7 mg/l (minimum acceptable value)

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
other: Cv
Remarks:
Cv%=|Tt48−Tt0|/Tt48 ×100 Tt48 is the titer determined at 48 hours, Tt 0 is the titer determined at 0 hours
Effect conc.:
ca. 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat. (dissolved fraction)
Remarks:
3.08%
Basis for effect:
mobility
Details on results:
At the end of the definitive test, EC50 was calculated using the EPA software PROBIT. 95% confidence limits was also calculated. The PROBIT elaboration was attached to the raw data.
EC50: 8.752 mg/l (confidence limits 7.022 mg/l – 10.709 mg/l)

Any other information on results incl. tables

 Test Solution  

%Immobilization

(24 h)

 

%Immobilization

(48 h)

 Initial pH  Final pH  Initial Dissolved Oxygen  Final Dissolved Oxygen
 

C1

(500 mg/l)

 80 %  100 %  7.83  7.52  8.89  9.34
 

C2

(250 mg/l)

 50 %  90 %  7.82  7.43  8.56  8.90
 

C3

(125 mg/l)

 30 %  70 %  7.83  7.36  9.24  9.95
 

C4

(62.5 mg/l)

 10 % 30 %   7.92  7.29  9.60  9.52
 

C5

(31.3 mg/l)

 0 %  0 %  7.99  7.23  9.17  9.72
 Negative Control  0 % 0 %   8.03  7.62  8.78  9.11

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
the concentrations used in this test were: 3.2 mg/l – 1.8 mg/l – 1 mg/l – 0.56 mg/l – 0.32 mg/l. Results of the tests were reported in the control chart 775/GxP. The calculated EC50 resulted between 0.6 mg/l and 2.1 mg/l.
Conclusions:
According to OECD 202:2004, under the test conditions applied, the EC50 of the test substance is calculated as 8.752 mg/l.