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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
03.07.2017 - 19.09.20172017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report date:
2017

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Neutralisation product of iron oxide hydroxide, phosphoric acid, phosphonic acid, lithium hydroxide or lithium carbonate, D-Glucose, 4-O-β-D-galactopyranosyl-, hydrate (1:1) and amylopectin
EC Number:
942-575-9
Molecular formula:
not available
IUPAC Name:
Neutralisation product of iron oxide hydroxide, phosphoric acid, phosphonic acid, lithium hydroxide or lithium carbonate, D-Glucose, 4-O-β-D-galactopyranosyl-, hydrate (1:1) and amylopectin
Test material form:
solid: particulate/powder
Details on test material:
Batch Number: 21747297V0
Specific details on test material used for the study:
Batch no. 21747297V09

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
The test solutions were collected from each test loading rate and each control at test initiation and at test completion.

Test solutions

Vehicle:
no
Details on test solutions:
The test item is a difficult item as defined by the OECD Guideline 23 (2000) because it isa multi-component substance with inconsistent water solubility. In order to maximise the amount of test item in solution, the test solutions will be prepared as water solublefractions (WSF).
Water soluble fractions (WSFs) were prepared at 1.56, 3.13, 6.25, 12.5, 25, 50, and 100 mg/L nominal loading rates and tested alongside a negative control and a WSF control.
To prepare the WSFs, the test item was weighed and added directly to ~1L of nutrient medium in a 2L glass aspirator bottles. WSF solution volumes were then brought up to a total volume of 2L. Two litres of the WSF (method) control (0 mg/L) was prepared alongside the test item WSFs; however, no test item was added. Each solution was stirred on a stirplate using a magnetic stir bar to create a vortex (~10%) and covered with a Plexiglas sheet to avoid contamination and evaporation. Stirring occurred for approximately 20 hours at which time the solutions were drained through the side arm located at the bottom third of the aspirator bottle, discarding the first ~100 mL.
Approximately 500 mL of each test solution was collected in 600 mL glass beakers. Aseptic techniques were followed during WSF preparation.

Test organisms

Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
Source of test organisms: Canadian Phycological Culture Center, Waterloo, ON, Canada
Test organism identification: Strain CPCC #37, CP170302; In house culture: 2017Jul09A

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
22 - 23 °C
pH:
Initial: 6.7 - 7.5
Final: 6.9 - 7.7
Conductivity:
92 127 µS/cm
Nominal and measured concentrations:
Nominal test item loading rate: 1.56, 3.13, 6.25, 12.5, 25, 50, and 100 mg/L
Measured concentrations Lithium, Iron, Phosphorus (mean, mg/L): 0.05, 0.05, 0.46; 0.10, 0.08, 0.64; 0.20, 0.14, 1.09; 0.41, 0.27, 2.02; 0.84, 0.56, 3.97; 1.66, 1.05, 7.35;3.37, 2.25, 15.10
Details on test conditions:
Photo-period: Continuous (24-h light)
Light intensity: 5733 - 6452 lux
Test vessels: 250 mL Erlenmeyer flasks, mixed on a horizontal shaker at approximately 75 rpm
Control: Negative control, WSF method control
Replicates: 3 for each test solution and 6 for each control

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EL10
Effect conc.:
21.18 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOELR
Effect conc.:
12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Any other information on results incl. tables

72 -h ELR50 (cell yield): 49.8 mg/L

72 -h ELR10 (cell yield): 14.35 mg/L

72 -h NOELR (cellyield): 25 mg/L

Applicant's summary and conclusion