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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2011
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report date:
2011

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
April 13th, 2004 (MO-0196)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Copper di-D-gluconate
EC Number:
208-408-2
EC Name:
Copper di-D-gluconate
Cas Number:
527-09-3
Molecular formula:
C12H22CuO14
IUPAC Name:
copper(2+) bis(2,3,4,5,6-pentahydroxyhexanoate)
Test material form:
solid
Specific details on test material used for the study:
Storage: at room temperature and darkness (room 0.025, ecotox box)

Test organisms

Test organisms (species):
Daphnia magna

Test conditions

Test temperature:
20ºC +-2ºC
pH:
7.8 +- 0.2
Dissolved oxygen:
>7mg/L (90%)
Salinity:
Freshwater (ISO Medium)
Nominal and measured concentrations:
0.1-0.25-0.5-1-2 mg/L (4 replicates)
Details on test conditions:
Daphnias between 6 and 24hours old and produced from parthenogenesis reproducing brood female population.
Light: light/dark cycle 16/8 hours.
Validation of breeding: Quality control is performed 2 times per month with potassium dichromate according to control breeding sensibility.

Reference substance (positive control):
yes

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.74 mg/L
Details on results:
Confidence limits (95%): 0.67-0.80 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
EC50 (48h) =0.74 mg/L (<1mg/L). Substance classifies as Aq. Acute tox 1.

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