Copper di-D-gluconate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2011

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

April 13th, 2004 (MO-0196)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Copper di-D-gluconate

EC Number:

208-408-2

EC Name:

Copper di-D-gluconate

Cas Number:

527-09-3

Molecular formula:

C12H22CuO14

IUPAC Name:

copper(2+) bis(2,3,4,5,6-pentahydroxyhexanoate)

Test material form:

solid

Specific details on test material used for the study:

Storage: at room temperature and darkness (room 0.025, ecotox box)

Test organisms

Test organisms (species):

Daphnia magna

Test conditions

Test temperature:

20ºC +-2ºC

pH:

7.8 +- 0.2

Dissolved oxygen:

>7mg/L (90%)

Salinity:

Freshwater (ISO Medium)

Nominal and measured concentrations:

0.1-0.25-0.5-1-2 mg/L (4 replicates)

Details on test conditions:

Daphnias between 6 and 24hours old and produced from parthenogenesis reproducing brood female population.
Light: light/dark cycle 16/8 hours.
Validation of breeding: Quality control is performed 2 times per month with potassium dichromate according to control breeding sensibility.

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.74 mg/L

Details on results:

Confidence limits (95%): 0.67-0.80 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

EC50 (48h) =0.74 mg/L (<1mg/L). Substance classifies as Aq. Acute tox 1.