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EC number: 500-607-5
CAS number: 161278-17-7
The test item, EK195, elicited a mean tissue viability of 23.9% for
three minute contact and 6.7% for one hour contact and was predicted as
corrosive in the EpiDerm™ skin corrosivity test.
The aim of this study is to evaluate the skin corrosion potential of the
test item, EK195.
The test item was applied for three minutes and one hour to the EpiDerm™
three-dimensional human skin model. The model consisted of normal,
human-derived epidermal keratinocytes, which had been cultured on 0.6
cm2 inserts to form a multilayered, highly differentiated model of the
human epidermis with a functional multilayered stratum corneum. The cell viability
of the multi layers was determined by mitochondrial dehydrogenase
activity assessed by the reduction of MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to a
soluble, coloured, formazan product. The formazan produced was
quantified by spectrophotometric measurement. The prediction model uses
the percentage viability values (compared to negative control viability)
at three minute and one hour exposure times to identify corrosive and
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