Condensation products of N-(2-aminoethyl)octadecanamide and N-(2-aminoethyl)hexadecanamide with sebacic acid

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Kyoeisha Chemical co. Ltd. / 6021874
- Expiration date of the lot/batch: February 18, 2019
- Purity test date: February 18, 2017

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room tempertaur in original container away from heat and sunlight.
- Stability under test conditions:
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: none
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:

FORM AS APPLIED IN THE TEST (if different from that of starting material)

TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)

OTHER SPECIFICS:
- measurement of pH, osmolality, and precipitate in the culture medium to which the test chemical is added:
- other information:

Analytical monitoring:

yes

Details on sampling:

0,24, 48 and 72 hours

Vehicle:

yes

Details on test solutions:

The test solution swill be dependent on the solubility in water which is 0.00022mg/L so limit concentrations will be used

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: ATCC 22662
- Source (laboratory, culture collection): American Type Culture Collection, 10801, University of Boulevard, Manassas, Virginia, 20110-2209, USA
- Age of inoculum (at test initiation):
- Method of cultivation: Static conditions

ACCLIMATION
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed:

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Hardness:

not determined

Test temperature:

21 to 24 ± 2 °C

pH:

~7

Dissolved oxygen:

not deteremined

Salinity:

N/A

Conductivity:

not determined

Nominal and measured concentrations:

nominal concentration based on water solubility of 0.00022mg/L

Details on test conditions:

Additional to the control (without test item), the main study will be conducted to determine the effect of at least five test item concentrations on the growth of the alga. The concentrations will be selected based on the results of the range finding study in a geometric series with a separation factor not exceeding 3.2. Selected concentrations will be added through study plan amendment. There will be three replicates for treatment groups and six replicates for control groups.

Reference substance (positive control):

yes

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

ca. 0 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

EC50 value has been takne as the water solubilty for the substance

Results with reference substance (positive control):

The algal growth inhibition test with the positive control is carried out twice a year to ascertain the suitability, validity and reliability of laboratory test condition and the response of the test system.

Validity criteria fulfilled:

yes

Conclusions:

EC50 is deteremined to be <0.00022mg/l, the limit of water solubilty due to the limit of detection.

Description of key information

The EC50 is below 0.00022mg/L which is the value for water solubilty from the lowest detection limit.

Key value for chemical safety assessment

EC50 for freshwater algae:

0 mg/L