Registration Dossier

Administrative data

Description of key information

Skin Irritation:

Based on the available data for the for the experimental in vitro study for 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] and structurally similar read across substances and applying the weight of evidence approach, it can be concluded that the target chemical will also tend to behave in a similar that of the read across substances. Therefore, 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] was estimated to be not irritating to skin. Comparing the above annotations with the criteria of CLP regulation, 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] can be classified under the category “Not Classified”.

Eye Irritation:

Based on the available data for the target and structurally similar read across substances and applying the weight of evidence approach, it can be concluded that the target chemical will also tend to behave in a similar that of the read across substances. Therefore, 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] was estimated to be not irritating to eyes. Comparing the above annotations with the criteria of CLP regulation, 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] can be classified under the category “Not Classified”.

Key value for chemical safety assessment

Skin irritation / corrosion

Link to relevant study records
Reference
Endpoint:
skin irritation: in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Data is from experimental study report
Qualifier:
according to
Guideline:
OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)
Principles of method if other than guideline:
The purpose of this study was to assess potential for the test article to be dermal irritants. The dermal irritation potential of test article may be predicted by measurement of their cytotoxic effect, as reflected in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, in the MatTek EpiDerm™ model.
GLP compliance:
no
Specific details on test material used for the study:
- Name of test material (IUPAC name): 1,4-phenylene bis[(4-phenoxyphenyl)-methanone]
- Molecular formula: C32H22O4
- Molecular weight: 470.5218 g/mol
- Smiles : c1ccc(cc1)Oc2ccc(cc2)C(=O)c3ccc(cc3)C(=O)c4ccc(cc4)Oc5ccccc5
- InChI : 1S/C32H22O4/c33-31(25-15-19-29(20-16-25)35-27-7-3-1-4-8-27)23-11-13-24(14-12-23)32(34)26-17-21-30(22-18-26)36-28-9-5-2-6-10-28/h1-22H
- Substance Type: Organic
- Physical State: Solid
- Physical Appearance: White solid powder
Test system:
human skin model
Source species:
human
Cell type:
non-transformed keratinocytes
Cell source:
other: as provided by MatTek In Vitro Life Science Laboratories, Bratislava, Slovakia
Source strain:
other: Not applicable
Details on animal used as source of test system:
- Description of the cell system used:The normal human-derived keratinocytes were cultured at the air-liquid interface in a chemically defined medium on a permeable polycarbonate insert (surface 0.5 cm2). They were cultured in chemically defined serum free medium to form a multi-layered epithelium similar to that found in native epidermis. Each lot of tissues was Quality Assured by MatTek according to specific QC standards including: histology, tissue viability (MTT mean optical density), reproducibility (SD) and tissue thickness.Test System IdentificationAll of the EpiDerm™ 3-dimensional human tissues used in this study were identified by the date of arrival and the lot number. Certificate of Analysis for the tissues are included in this report. Tissue plates were appropriately labeled with study information.
Justification for test system used:
The 3-Dimensional Human Dermal Epithelial Model (EpiDerm™, MatTek, In Vitro Life Science Laboratories, Bratislava, Slovakia) is made up of normal human keratinocytes in serum free medium. The cells form an epithelial tissue that consists of organized basal, spinous, granular, and cornified layers analogous to those found in vivo. The EpiDerm™ model also contains epidermis-specific differentiation markers such as pro-filaggrin, the K1/K10 cytokeratin pair, involucrin, and type I epidermal transglutaminase, as well as keratohyalin granules, tonofilament bundles, desmosomes, and a multi-layered stratum corneum containing intercellular lamellar lipid layers arranged in patterns characteristic of in vivo epidermis. Each lot of tissues was Quality Assured by MatTek, Inc. according to specific QC standards including: histology (cell layers), tissue viability (MTT mean optical density) and reproducibility (SD). Tissue plates were appropriately labeled with study information. Bias was not a factor in this test system.
Vehicle:
unchanged (no vehicle)
Details on test system:
The tissues were exposed to the test article neat (undiluted) on April 25, 2018 (Run 1 of 1). EpiDerm™ tissues were purchased from MatTek. Quality control of the tissues was performed by MatTek and the Certificate of Analysis (CoA) for the tissues is provided and is kept in the study binder. Tissues were exposed for approximately 1 hour, with 35 minutes in an approximately 37°C, 5% CO2 humidified incubator and the remaining 25 minutes at room temperature. Following the exposure time, the tissues were rinsed and placed in fresh media for approximately 24 hours. The media was then changed again and the tissues were incubated in fresh media for another ~18 hours for a total of approximately 42 hour post-exposure recovery period. The tissue viability was then assessed by MTT assay. The tissue CoA was used instead of verification of barrier properties of the tissue.MTT and Color Pre-testsPretesting has actually been conducted for all chemicals, although the first intitial 8 test chemicals a pretesting was not conducted (for skin).MTT AssayFollowing the rinsing period, the MTT assay was performed by transferring the tissues to 24-well plates containing 300 µL MTT medium (1.0 mg/mL). After 3 hours MTT incubation at approximately 37°C, approximately 5% CO2 in a humidified incubator, the blue formazan salt was extracted by submerging tissues in 2 mL isopropanol in a 24-well plate. The extraction time was approximately 3 hours with gentle shaking. The optical density of the extracted formazan (200 µL/well of a 96-well plate) was determined using a Thermo Scientific Multiskan FC Microplate Photometer at 570 nm. Relative cell viability is calculated for each tissue as % of the mean negative control tissues.Evaluation of Test Article in the Cell Models:1. Cell system: Upon receipt, the MatTek EpiDerm™ tissue cultures were placed in 0.9 mL of fresh Maintenance medium (in a 6-well plate). The culture inserts are incubated for ~one hour. The tissues were then transferred to 6-well plates containing 0.9 mL fresh Maintenance medium and they were incubated overnight (18 ± 3 hrs) at ~37°C, 5% CO2 in a humidified incubator.2. Control and Test Article Exposures: On the day of dosing, the tissues are then removed from the incubator and the controls and the test article are applied topically to tissues by pipette(liquid) Tissues were exposed to controls and the test article for one hour, with ~35 minutes in a 37°C, 5% CO2 humidified incubator and the remaining 25 minutes at room temperature.a) Controls30 µL of negative control DPBS and 30 μL of the positive control 5% SDS was applied topically to the tissue and gently spread by placing a nylon mesh on the apical surface of each tissue, if necessary.b) Test Articles 25 mg of the test article was applied topically to the tissue 3. Post-exposure treatmentAfter the 1 hour exposure, the tissues were rinsed 15 times with sterile DPBS. After the 15th rinse from washing bottle, each insert wasw completely submerge 3 times in 150 ml DPBS. The apical surface was gently blotted with a cotton swab. The tissues were placed in 0.9 mL of fresh Maintenance medium (6-well plate) for 24 ± 2 hours. After this initial ~24 hour incubation, the tissues were placed in 6-well plates containing 0.9 mL fresh Maintenance medium and incubated for another 18 ± 3 hours, for a total of an approximately 42 hour post-exposure incubation.RECONSTRUCTED HUMAN EPIDERMIS (RHE) TISSUE- Model used: The EpiDerm™ 3 dimensional human tissue model- Tissue Lot number(s): 26459- Date of initiation of testing: 6/08/2017TEMPERATURE USED FOR TEST SYSTEM- Temperature used during treatment / exposure: 37°C- Temperature of post-treatment incubation (if applicable): 37°CREMOVAL OF TEST MATERIAL AND CONTROLS-Volume and number of washing steps: The test substance was rinsed from the tissues with sterile DPBS by filling and emptying the tissue insert 15 times to remove any residual test material. This was followed by completely submerge the insert 3 times in 150 ml DPBS.MTT DYE USED TO MEASURE TISSUE VIABILITY AFTER TREATMENT / EXPOSURE- MTT concentration: 300 µL MTT medium (1.0 mg/mL).- Incubation time: After 3 hours- Spectrophotometer: Synergy H4 spectrophotometer - Wavelength: 570 nm- Filter: No data- Filter bandwidth: No data- Linear OD range of spectrophotometer: No dataNUMBER OF REPLICATE TISSUES: 3CALCULATIONS and STATISTICAL METHODSAll data were background subtracted before analysis. MTT data are presented as % viable compared to negative control. Data were generated as follows: MTT AssayBlanks:·        The optical density (OD) mean from all replicates for each plate (ODblank). Negative Controls (NC):·        The blank corrected value was calculated: ODNC= ODNCraw– ODblank. ·        The OD mean per NC tissue was calculated. ·        The mean OD for all tissues corresponds to 100% viability. ·        The mean, standard deviation (SD), standard error of the mean (SEM) and the percent coefficient of variation (% CV) was calculated. Positive Control (PC):·        Calculate the blank corrected value: ODPC= ODPCraw– ODblank. ·        The OD mean per PC tissue was calculated. ·        The viability per tissue was calculated: %PC = [ODPC/ mean ODNC] x 100. ·        The mean viability for all tissues was calculated: Mean PC = Σ %PC / number of tissues. ·        The standard deviation (SD), standard error of the mean (SEM) and the percent coefficient of variation (% CV) was calculated. Tested compound :·        Calculate the blank corrected value ODTT= ODTTraw– ODblank. ·        The OD mean per tissue was calculated. ·        The viability per tissue was calculated: %TT = [ODTT/ mean ODNC] x 100. ·        The mean viability for all tissues was calculated: Mean TT = Σ %TT / number of tissues. ·        The standard deviation (SD), standard error of the mean (SEM) and the percent coefficient of variation (% CV) was calculated. Data Correction Procedure for MTT Interfering Compounds (if applicable)True viability = Viability of treated tissue – Interference from test article = ODtvt– ODktwhere ODkt= (mean ODtkt– mean ODukt).ODtvt= optical density of treated viable tissueODkt= optical density of killed tissuesODtkt= optical density of treated killed tissueODukt= optical density of untreated killed tissue (NC treated tissue) Data Correction Procedure for Colored Compounds (if applicable)True viability = Viability of treated tissue incubated in MTT media – Viability of treated tissue incubated in media without MTT = ODtvt– ODvt.ODtvt= optical density of treated viable tissue incubated in MTT mediaODvt= optical density of viable tissues incubated in media alone - Evaluation of data The results of the assay was evaluated and compared to negative control. Table: Criteria for in vitro Interpretation: In VitroResults In VivoPredictionMean tissue viability ≤50% Irritant (I), R38Mean tissue viability >50% Non-irritant (NI)- Assay quality controls- Negative Controls (NC)The Dulbecco’s phosphate buffered saline (DPBS) was used as a NC. The assay passed all acceptance criteria if the ODs of the negative control exposed tissues were between ≥0.8 and ≤2.8.  - Positive Controls (PC)5% solution of sodium dodecyl sulfate was used as a PC. The assay is meeting the acceptance criteria if the viability of the PC is ≤20% of the negative control.   - Standard Deviation (SD)The standard deviation (SD) calculated from individual percent tissue viabilities of the test article exposed replicates was ≤18.
Control samples:
yes, concurrent negative control
yes, concurrent positive control
Amount/concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit):25 mg
- Concentration (if solution): neat

VEHICLE (Not used)
- Amount(s) applied (volume or weight with unit): none
- Concentration (if solution): none
- Lot/batch no. (if required): none
- Purity: none

NEGATIVE CONTROL
- Amount(s) applied (volume or weight): 30 µL sterile DPBS
- Concentration (if solution): neat

POSITIVE CONTROL
- Amount(s) applied (volume or weight): 30 µL
- Concentration (if solution): 5% solution of sodium dodecyl sulfate
Duration of treatment / exposure:
The exposure times were approximately 1 hour, with ~35 minutes exposure in the incubator and ~25 minutes at room temperature.
Duration of post-treatment incubation (if applicable):
For a total of an approximately 42 hour post-exposure incubation.
Number of replicates:
3 tissues were used for test compound and control.
Irritation / corrosion parameter:
% tissue viability
Run / experiment:
Run 1
Value:
90.8
Vehicle controls validity:
not specified
Negative controls validity:
valid
Positive controls validity:
valid
Remarks on result:
other: Mean of OD 1.806; Not irritating
Other effects / acceptance of results:
The MTT data show the assay quality controls were met.
Standard Deviation (SD)The standard deviation (SD) calculated from individual percent tissue viabilities of the test article exposed replicates was >18
Interpretation of results:
other: Not irritating
Conclusions:
The dermal irritation potential of test article was determined according to the OECD 439 test guideline followed for this study. The Mean % tissue viability compared to negative control (n=3) of the test substance 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] (CAS No. 54299-17-1) was determined to be 90.8 %. Thus, substance 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] (CAS No. 54299-17-1) was considered to be not irritating to the human skin.
Executive summary:

The dermal irritation potential of test article was determined according to the OECD 439 test guideline for this study. The MatTek EpiDerm™ model was used to assess the potential dermal irritation of the test article by determining the viability of the tissues following exposure to the test article via MTT. Tissues were exposed to the test article and controls for ~one hour, followed by a 42 hour post-exposure recovery period. The viability of each tissue was determined by MTT assay. 

The MTT data show the assay quality controls were met and passed the acceptance of criteria.

The Mean % tissue viability compared to negative control (n=3) of the test substance 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] (CAS No. 54299-17-1) was determined to be 90.8 %.

Hence, under the current experimental test conditions it was concluded that test substance 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] (CAS No. 54299-17-1)was considered to be not irritating to human skin and can thus be classified as “Not Classified'' as per CLP Regulation.

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (not irritating)

Eye irritation

Link to relevant study records
Reference
Endpoint:
eye irritation: in vivo
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Weight of evidence approach based on the similar chemicals
Justification for type of information:
Weight of evidence approach based on the similar chemicals
Reason / purpose:
read-across: supporting information
Reason / purpose:
read-across: supporting information
Qualifier:
according to
Guideline:
other: weight of evidence based on similar chemicals
Principles of method if other than guideline:
The weight of evidence report has been prepared based on the read across substances identified based on structural and functional similarity to assess the ocular irritation potential of 1,4-phenylene bis[(4-phenoxyphenyl)-methanone]
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (IUPAC name): 1,4-phenylene bis[(4-phenoxyphenyl)-methanone]
- Molecular formula: C32H22O4
- Molecular weight: 470.5218 g/mol
- Smiles : c1ccc(cc1)Oc2ccc(cc2)C(=O)c3ccc(cc3)C(=O)c4ccc(cc4)Oc5ccccc5
- InChI : 1S/C32H22O4/c33-31(25-15-19-29(20-16-25)35-27-7-3-1-4-8-27)23-11-13-24(14-12-23)32(34)26-17-21-30(22-18-26)36-28-9-5-2-6-10-28/h1-22H
- Substance Type: Organic
- Physical State: Solid
Species:
rabbit
Strain:
not specified
Vehicle:
unchanged (no vehicle)
Controls:
not specified
Amount / concentration applied:
100 mg undiluted
Duration of treatment / exposure:
24 hours
Observation period (in vivo):
7 days
Duration of post- treatment incubation (in vitro):
no data available
Number of animals or in vitro replicates:
1. 6
2. 5
Details on study design:
The study is based on weight of evidence approach from the read across values
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
7 d
Score:
0
Reversibility:
not specified
Remarks on result:
no indication of irritation
Irritant / corrosive response data:
no signs of irritation observed
Interpretation of results:
other: not irritating
Conclusions:
Based on the available data for the similar read across substances and applying the weight of evidence approach, it can be concluded that the target chemical will also tend to behave in a similar that of the read across substances. Therefore, 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] was estimated to be not irritating to eyes. Comparing the above annotations with the criteria of CLP regulation, 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] can be classified under the category “Not Classified”.
 
Executive summary:

Based on the available studies for the similar read across chemicals, weight of evidence approach was applied to assess the ocular irritation potential of 1,4-phenylene bis[(4 -phenoxyphenyl)-methanone].

The ocular irritation potential of the similar test chemical was assessed in albino rabbits.

The study was performed as per Draize method. 100 mg of the undiluted test chemical was instilled in to the eyes of 6 rabbits. The eyes remained unwashed throughout the test. The rabbits were observed for signs of irritation and scored at 1,2,3,4 and 7 days.

No signs of irritation observed till 7 days after exposure to 100% undiluted test chemical. The average scores per day till 7 days of observation were 0.0.

Hence, the test chemical can be considered to be not irritating to rabbit eyes.

This result is supported by the Draize study conducted for the other similar chemical to determine its ocular irritation potential. 100 mg of the undiluted test chemical was instilled in to the eyes of 5 rabbits. The eyes remained unwashed throughout the test. The rabbits were observed for signs of irritation and scored at 1,2,3,4 and 7 days.

No signs of irritation observed till 7 days after exposure to 100% undiluted test chemical. The average scores per day till 7 days of observation were 0.0.

Hence, the test chemical can be considered to be not irritating to rabbit eyes.

Based on the available data for the similar read across substances and applying the weight of evidence approach, it can be concluded that the target chemical will also tend to behave in a similar that of the read across substances. Therefore, 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] was estimated to be not irritating to eyes. Comparing the above annotations with the criteria of CLP regulation, 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] can be classified under the category “Not Classified”.

 

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (not irritating)

Respiratory irritation

Endpoint conclusion
Endpoint conclusion:
no study available

Additional information

Skin Irritation:

Based on the available studies for the target as well as its structurally similar read across chemicals, studies have been conducted in vitro and in vivo to assess the dermal irritation potential of 1,4-phenylene bis[(4-phenoxyphenyl)-methanone].

 

The dermal irritation potential of test article was determined according to the OECD 439 test guideline for this study. The MatTek EpiDerm™ model was used to assess the potential dermal irritation of the test article by determining the viability of the tissues following exposure to the test article via MTT. Tissues were exposed to the test article and controls for ~one hour, followed by a 42 hour post-exposure recovery period. The viability of each tissue was determined by MTT assay.  The MTT data show the assay quality controls were met and passed the acceptance of criteria. The Mean % tissue viability compared to negative control (n=3) of the test substance 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] (CAS No. 54299-17-1) was determined to be 90.8 %. Hence, under the current experimental test conditions it was concluded that test substance 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] (CAS No. 54299-17-1)was considered to be not irritating to human skin and can thus be classified as “Not Classified'' as per CLP Regulation.

 

This above in vitro result is supported by a study designed and conducted to determine the dermal reaction profile of the structurally similar test chemical in Sprague Dawley rats. The study was performed as per OECD Guidelines 402 and complying to the GLP procedures. Ten rats (5 male and 5 female) were used for conducting dermal irritation /corrosion study. The animals were kept in their cages for at least 5 days prior to administration for acclimatization to the laboratory condition and after acclimatization period, animals were randomly selected. Approximately 24 hours before application, the hair of each rat was closely clipped from the trunk (dorsal surface and sides from scapular to pelvic area) with an electric clipper, so as to expose at least 10% of the body surface area. The test item was moistened with distilled water. The test item was applied onto the exposed skin of the animal, taking care to spread the test item evenly over the entire area of approximately 10% of the total body surface area or as much of the area as can reasonably be covered. The test item was held in contact with the skin using a porous gauze dressing and non-irritating tape around the animal to cover the exposure site for first 24 hours exposure period. Elizabethan collar was placed on each animal for first 24 hours after application of the test item. These collars prevent ingestion of test item. Following 24 hours of exposure, the wrapping was removed and the test site wiped free of excess test item. Distilled water was used to remove residual test item. The test item was applied to shorn skin of 5 male and 5 female animals at 2000 mg/kg body weight. Administration of the test item at 2000 mg/kg did not result in any skin reaction at the site of application during the study period of 14 days. Administration of the test item did not result in any signs of toxicity and mortality during the study period of 14 days. The overall irritation score of the substance was determined to be 0 and no erythema and edema (skin irritation) were found at the end of 14 days observation period after patch removal.

Hence, it was concluded that the test chemical was Non-Irritating to the skin of Sprague Dawley rats under the experimental conditions tested and classified as “Category- Not Classified” as per CLP Classification.

 

The above results are further supported by an OECD 404 study performed to determine the irritation potential of the other structurally similar test chemical. 3 young adult male New Zealand White rabbits weighing 2.2 to 2.3 kg were used for the study. They were housed individually in metal cages, were kept at a constant room temperature of 20±3 °C, at a relative humidity of 30-70%, and on a 12 hours light cycle day. The animals received ad libitum standard pelleted rabbit food (Nafag No. 814; Gossau, Switzerland) and water. Prior to treatment they were acclimatized for a minimum of 5 days. The test article was applied as a liquid formulation containing 21.3% test chemical in water. About 24-hours before treatment an area of about 6 sq. cm was shaved on both flanks of each rabbit. Gauze patch of 2.5 x 2.5 cm covered with 0.5 mL of the test material was applied to one flank and a control patch to the contralateral flank. The patches were covered with an impermeable material and were fastened to the body of the rabbit with adhesive tape. The duration of treatment was four hours. The scoring of skin reaction was performed 1, 24, 48 and 72 hours after removal of the dressing. These scores were used in calculating the respective mean values for each type of lesion. At one hour, erythema was observed in each animal (one with grade 2; two with grade 1) as was grade 1 edema. At 24-hours, no signs of erythema or edema were observed. The calculated primary irritation index was 0.00 (max.8.0).The test chemical did not cause any staining to skin. Also, no signs of corrosion were observed throughout the study. Based on the scores and observations, the test chemical can be considered to be not irritating to skin.

Based on the available data for the target and structurally similar read across, it can be concluded that the target chemical will also tend to behave in a similar that of the read across substances. Therefore, 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] was estimated to be not irritating to skin. Comparing the above annotations with the criteria of CLP regulation, 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] can be classified under the category “Not Classified”.

Eye Irritation

Based on the available studies for the similar read across chemicals, weight of evidence approach was applied to assess the ocular irritation potential of 1,4-phenylene bis[(4 -phenoxyphenyl)-methanone].

The ocular irritation potential of the similar test chemical was assessed in albino rabbits.

The study was performed as per Draize method. 100 mg of the undiluted test chemical was instilled in to the eyes of 6 rabbits. The eyes remained unwashed throughout the test. The rabbits were observed for signs of irritation and scored at 1,2,3,4 and 7 days.

No signs of irritation observed till 7 days after exposure to 100% undiluted test chemical. The average scores per day till 7 days of observation were 0.0.

Hence, the test chemical can be considered to be not irritating to rabbit eyes.

This result is supported by the Draize study conducted for the other similar chemical to determine its ocular irritation potential. 100 mg of the undiluted test chemical was instilled in to the eyes of 5 rabbits. The eyes remained unwashed throughout the test. The rabbits were observed for signs of irritation and scored at 1,2,3,4 and 7 days.

No signs of irritation observed till 7 days after exposure to 100% undiluted test chemical. The average scores per day till 7 days of observation were 0.0.

Hence, the test chemical can be considered to be not irritating to rabbit eyes.

Based on the available data for the similar read across substances and applying the weight of evidence approach, it can be concluded that the target chemical will also tend to behave in a similar that of the read across substances. Therefore, 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] was estimated to be not irritating to eyes. Comparing the above annotations with the criteria of CLP regulation, 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] can be classified under the category “Not Classified”.

 

Justification for classification or non-classification

The results of the experimental in vitro studies for 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] and from the structurally similar read across substances indicate a possibility that 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] can be not irritating to skin and eyes.

Hence by applying the weight of evidence approach, 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] can be considered to be not irritating to skin and eyes. It can be classified under the category “Not Classified” as per CLP regulation.