Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Principles of method if other than guideline:
Acute Immobilization Study of 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] on Daphnia magna in a Semi-Static System for 48 hrs. Test performed according to the OECD guideline 202.
GLP compliance:
no
Specific details on test material used for the study:
- Name of test material (IUPAC name): 1,4-phenylene bis[(4-phenoxyphenyl)-methanone]
- Molecular formula: C32H22O4
- Molecular weight: 470.5218 g/mol
- Smiles : c1ccc(cc1)Oc2ccc(cc2)C(=O)c3ccc(cc3)C(=O)c4ccc(cc4)Oc5ccccc5
- InChI : 1S/C32H22O4/c33-31(25-15-19-29(20-16-25)35-27-7-3-1-4-8-27)23-11-13-24(14-12-23)32(34)26-17-21-30(22-18-26)36-28-9-5-2-6-10-28/h1-22H
- Substance Type: Organic
- Physical State: Solid
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The test substance was poorly soluble in water. Therefore, the test solution was prepared by dissolving 500 mg of the test substance in 500 ml of ADaM’s media. Kept it for 72 hrs stirring, after stirring stock was filtered and analytically detected and the stock concentration was found to be 0.2046 mg/L.As it is stock taken and set up limit test on this specific value.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
24 and 48 hrs
Hardness:
144.5 mg of CaCO3)
Test temperature:
20±2 °C
pH:
7.2
Nominal and measured concentrations:
0.2046 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.205 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: No effects were observed

Table 1: Immobility

Nominal

Concentration

(mg/l)

Number of

Daphnids

Immobility

0 hrs

24 hrs

48 hrs

0.2046

10

-

-

-

0.2046

10

-

-

-

Any Abnormal Behaviour(if any)

 

No observed effects

No observed effects

No observed effects

Table 2: pH and oxygen content

Nominal

Concentration

(mg/l)

Number of

Daphnids

pH value

0 hrs

48 hrs

 

control

10

7.4

7.6

7.8

0.2046

10

7.5

7.7

7.9

0.2046

10

7.5

7.7

7.9

Nominal

Concentration

(mg/l)

Number of

Daphnids

DO(mg/L)

 

0 hrs

48 hrs

Control

10

6.0

5.8

5.8

0.2046

10

6.1

5.9

5.9

0.2046

10

6.1

5.9

5.9

Table 3: Test temperature

Nominal

Concentration

(mg/l)

Number of

Daphnids

Temperature

 

0 hrs

48 hrs

Control

10

20.1

 

20.1

0.2046

10

20.1

 

20.1

0.2046

10

20.1

 

20.1

Validity criteria fulfilled:
yes
Remarks:
1. In the control,0 percent of the daphnids observed immobilized. 2. The dissolved oxygen concentration at the end of the test should be 5.8 mg/l in control and 5.9 in test vessels
Conclusions:
After the exposure of chemical with daphnia magna for period of 48 hrs, no effects were observed on the mobility of daphnia magna. Thus the EC0 was determine to be 0.2046 mg/l.
Executive summary:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for 1,4-phenylene bis[(4-phenoxyphenyl)-methanone]. The test substance was poorly soluble in water. Therefore, the test solution was prepared by dissolving 500 mg of the test substance in 500 ml of ADaM’s media. Kept it for 72 hrs stirring, after stirring stock was filtered and analytically detected and the stock concentration was found to be 0.2046 mg/L.As it is stock taken and set up limit test on this specific value. Limit test were performed and test Daphnia magna were exposed to these concentration for 48 hours. Test considers to be valid as it fulfills the validity criteria. In the control, 0 percent of the Daphnis observed immobilized. The dissolved oxygen concentration at the end of the test should be 5.8 mg/l in control and 5.9 in test vessel. After the exposure of chemical with daphnia magna for period of 48 hrs, no effects were observed on the mobility of daphnia magna. Thus the EC0 was determining to be 0.2046 mg/l. As no effects were observed on the mobility of daphnia magna, this chemical considers to be nontoxic and not classified as per the CLP classification criteria.

Description of key information

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for 1,4-phenylene bis[(4-phenoxyphenyl)-methanone]. The test substance was poorly soluble in water. Therefore, the test solution was prepared by dissolving 500 mg of the test substance in 500 ml of ADaM’s media. Kept it for 72 hrs stirring, after stirring stock was filtered and analytically detected and the stock concentration was found to be 0.2046 mg/L.As it is stock taken and set up limit test on this specific value. Limit test were performed and test Daphnia magna were exposed to these concentration for 48 hours. Test considers to be valid as it fulfills the validity criteria. In the control, 0 percent of the Daphnis observed immobilized. The dissolved oxygen concentration at the end of the test should be 5.8 mg/l in control and 5.9 in test vessel. After the exposure of chemical with daphnia magna for period of 48 hrs, no effects were observed on the mobility of daphnia magna. Thus the EC0 was determining to be 0.2046 mg/l. As no effects were observed on the mobility of daphnia magna, this chemical considers to be nontoxic and not classified as per the CLP classification criteria.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
0.205 mg/L

Additional information

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for 1,4-phenylene bis[(4-phenoxyphenyl)-methanone]. The test substance was poorly soluble in water. Therefore, the test solution was prepared by dissolving 500 mg of the test substance in 500 ml of ADaM’s media. Kept it for 72 hrs stirring, after stirring stock was filtered and analytically detected and the stock concentration was found to be 0.2046 mg/L.As it is stock taken and set up limit test on this specific value. Limit test were performed and test Daphnia magna were exposed to these concentration for 48 hours. Test considers to be valid as it fulfills the validity criteria. In the control, 0 percent of the Daphnis observed immobilized. The dissolved oxygen concentration at the end of the test should be 5.8 mg/l in control and 5.9 in test vessel. After the exposure of chemical with daphnia magna for period of 48 hrs, no effects were observed on the mobility of daphnia magna. Thus the EC0 was determining to be 0.2046 mg/l. As no effects were observed on the mobility of daphnia magna, this chemical considers to be nontoxic and not classified as per the CLP classification criteria.