Reaction product of 2,3-epoxypropyl neodecanoate and Benzenesulfonic acid, C10-13-sec-alkyl derivatives

Endpoint summary

Description of key information

Short-term toxicity to aquatic invertebrates

The acute toxicity of 'Reaction product of 2,3-epoxypropyl neodecanoate and Benzenesulfonic acid, C10-13-sec-alkyl derivatives' to the freshwater invertebrate Daphnia magna was assessed according to the OECD Guideline 202 ("Daphnia sp., acute immobilisation test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008). The study was conducted at a GLP accredited laboratory and met the validity criteria described in the Guideline and is therefore considered to be reliable.

 

Due to the low aqueous solubility and complex nature of the test item, the test medium was prepared as a Water Accommodated Fraction (WAF). Following a preliminary range-finding test, 20 daphnids (four replicates of five animals) were exposed to WAFs of the test item over a range of nominal loading rates of 1.0, 1.8, 3.2, 5.6 and 10 mg/L for 48 hours at a temperature of approximately 20 to 22°C under static test conditions. The number of immobilised daphnids and any adverse reactions to exposure were recorded after 24 and 48 hours.

 

Chemical analysis of the fresh test preparations at 0 hours showed measured test concentrations to range from 2.3 to 9.3 mg/L. Chemical analysis of the aged test preparations at 48 hours showed measured test concentrations to range from 2.5 to 8.6 mg/L. The dissolved test item may have been one or several components of the test item. Given that the toxicity cannot be attributed to a single component or a mixture of components, but to the test item as a whole, the results were based on nominal loading rates only.

The study resulted in 48-hour EL50, NOELR and LOELR values of 6.9, 5.6 and 10 mg/L, respectively.

Toxicity to aquatic algae and cyanobacteria

The toxicity of 'Reaction product of 2,3-epoxypropyl neodecanoate and Benzenesulfonic acid, C10-13-sec-alkyl derivatives' to the freshwater green algaPseudokirchneriella subcapitatawas assessed according to the OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test" referenced as Method C.3 of Commission Regulation (EC) 761/2009). The study was conducted at a GLP accredited laboratory and met the validity criteria described in the Guideline and is therefore considered to be reliable.

 

Due to the low aqueous solubility and complex nature of the test item, the test medium was prepared as a Water Accommodated Fraction (WAF). Following a preliminary range-finding test, Pseudokirchneriella subcapitatawas exposed to Water Accommodated Fractions (WAFs) of the test item over a range of nominal loading rates of 1.0, 3.2, 10, 32 and 100 mg/L (3 replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 ±1°C. Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a haemocytometer and light microscope.

Chemical analysis of the fresh test preparations at 0 hours showed measured test concentrations to range from 0.70 to 60 mg/L. Measured test concentrations in the range of 0.35 to 59 mg/L were obtained at 72 hours. The dissolved test item may have been one or several components of the test item. Given that the toxicity cannot be attributed to a single component or a mixture of components, but to the test item as a whole, the results were based on nominal loading rates only.

 

The study resulted in 72-hour EL50, NOELR and LOELR values of 31, 3.2 and 10 mg/L, respectively based on growth rate and 72-hour EL50, NOELR and LOELR values of 5.7, 1 and 3.2 mg/L, respectively based on yield.