Registration Dossier
Registration Dossier
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EC number: 293-391-4 | CAS number: 91079-06-0
Environmental fate & pathways
Biodegradation in water: screening tests
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Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- The substance is considered very similar to the analogue derived from vegetable oils (see section 13).The latter was tested carring out a OECD 301B study, together with the other representative samples of sulfated oils, which this read-across record refers to. Unfortunately, as stated by the Laboratory "For 7 of the 8 substances we could determine the carbon content, so that we are able to conduct the studies acc. to OECD 301 B. Only for CNH [Oils, vegetable, sulfated, ammonium salts - Editor's Note], we could not determine the carbon content because flakes were formed during analysis" (ref.: email, 2012-02-12, from RTC Lab). For this reason, an exeperimental study is not available, but given the structural similarities of the substance against the other representative samples of sulphated oils, it was not considered necessary to run again the study. Fo rmore details on the structural similarities of the sulphated oils, refer to section 13.2.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- no
- Specific details on test material used for the study:
- It has been reported the test material correspondent to the analogue substance "Oils, vegetable, sulfated, ammonium salts", since animal and vegetable oils are considered to be very similar, and not only for the kind of salification. Anyway, seven test materials, analogues of the substance, were analysed:
Oils, fish, sulfated, sodium salts (P2)
Rape oil, sulfated, sodium salt (CP12)
Rape oil, sulfated, sodium salt (C5)
Rape oil, sulfated, sodium salt (CSO3)
Rape oil, sulfated, sodium salt (CSO5)
Castor oil, sulfated, sodium salt (R3)
Rape oil, sulfated, compd. with ethanolammine (CTEA) - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal STP, Hildesheim, D-31137
- Reason of selection: Non adapted activated sludge from the stated sewage plant is well suited as it receives predominantly municipal sewage
- Date of receipt: 2012-04-19
- Storage conditions: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2-4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition for 6 days by aeration with CO2 free air until test start.
- Storage length: 6 days
- Concentration of sludge: 10 mg/L
- Initial cell/biomass concentration: 10E+7 - 10E+8 CFU/L - Initial conc.:
- 15 mg/L
- Based on:
- test mat.
- Remarks:
- CP12
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
- Remarks:
- C5
- Initial conc.:
- 15 mg/L
- Based on:
- test mat.
- Remarks:
- CSO3
- Initial conc.:
- 15 mg/L
- Based on:
- test mat.
- Remarks:
- CSO5
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
- Remarks:
- P2
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
- Remarks:
- R3
- Initial conc.:
- 15 mg/L
- Based on:
- test mat.
- Remarks:
- CTEA
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: As prescribed by OECD TG 301B
- Solubilising agent (type and concentration if used): None
- Test temperature: 21.0 - 23.5 deg C
- pH: 7.8 - 7.9
- pH adjusted: No
- Aeration of dilution water: Yes - 30 - 100 mL/minute
- Continuous darkness: Yes - contained in brown glass bottles
TEST SYSTEM
- Culturing apparatus: Brown glass incubation bottles, 5 L capacity
- Number of culture flasks/concentration: 2 x blank control; 2 x test substance; 1 x inhibition (toxicity) control; 1 x reference substance
- Method used to create aerobic conditions: Carbon dioxide free air at 30 - 100 mL/minute flow rate
- Measuring equipment: Barium hydroxide traps
- Details of trap for CO2 and volatile organics if used: 3 serial scrubbing bottles filled with 100 mL 0.0 125 mol/L barium hydroxide solution for the adsorption of carbon dioxide from biodegradation proces ses. At intervals the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined by titration and used for the calculation of the produced carbon dioxide. After each sampling the other traps were moved forward and a new trap with fresh barium hydroxide solution was placed into the final position.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Toxicity control: Yes
- Other: Positive control - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 61
- Sampling time:
- 28 d
- Remarks on result:
- other: test mat. CP12
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 85
- Sampling time:
- 28 d
- Remarks on result:
- other: test mat. C5
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 66
- Sampling time:
- 28 d
- Remarks on result:
- other: test mat. CSO3
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 63
- Sampling time:
- 28 d
- Remarks on result:
- other: test mat. CSO5
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 64
- Sampling time:
- 28 d
- Remarks on result:
- other: test mat. P2
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 62
- Sampling time:
- 28 d
- Remarks on result:
- other: test mat. R3
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 63
- Sampling time:
- 28 d
- Remarks on result:
- other: test mat. CTEA
- Details on results:
- The % degradation for the tested sulfated oils resulted to be >60%. The highest value obtained of 85% is related to the sample with the higher fraction of free fatty acids.
- Results with reference substance:
- 60% after 7 days, 86% after 28 days
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The biodegradability of the representative samples of the sulfated oils has been investigated using the CO2 evolution test method, performed according to OECD Test Guidelines OECD 301B.
The results obtained demonstrate that the substance is readily biodegradable. - Executive summary:
The biodegradability of the representative samples of the sulfated oils has been investigated using the CO2 eviolution test method, performed according to OECD Test Guidelines OECD 301B.
The results obtained demonstrate that the substance is readily biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal STP, Hildesheim, D-31137
- Reason of selection: Non adapted activated sludge from the stated sewage plant is well suited as it receives predominantly municipal sewage
- Date of receipt: 2012-04-19
- Storage conditions: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2-4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition for 6 days by aeration with CO2 free air until test start.
- Storage length: 6 days
- Concentration of sludge: 10 mg/L
- Initial cell/biomass concentration: 10E+7 - 10E+8 CFU/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: As prescribed by OECD TG 301B
- Solubilising agent (type and concentration if used): None
- Test temperature: 21.0 - 23.5 deg C
- pH: 7.8 - 7.9
- pH adjusted: No
- Aeration of dilution water: Yes - 30 - 100 mL/minute
- Continuous darkness: Yes - contained in brown glass bottles
TEST SYSTEM
- Culturing apparatus: Brown glass incubation bottles, 5 L capacity
- Number of culture flasks/concentration: 2 x blank control; 2 x test substance; 1 x inhibition (toxicity) control; 1 x reference substance
- Method used to create aerobic conditions: Carbon dioxide free air at 30 - 100 mL/minute flow rate
- Measuring equipment: Barium hydroxide traps
- Details of trap for CO2 and volatile organics if used: 3 serial scrubbing bottles filled with 100 mL 0.0125 mol/L barium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes. At intervals the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined by titration and used for the calculation of the produced carbon dioxide. After each sampling the other traps were moved forward and a new trap with fresh barium hydroxide solution was placed into the final position.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Toxicity control: Yes
- Other: Positive control - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 64
- Sampling time:
- 28 d
- Results with reference substance:
- 60% after 7 days, 86% after 28 days
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
- Executive summary:
Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal STP, Hildesheim, D-31137
- Reason of selection: Non adapted activated sludge from the stated sewage plant is well suited as it receives predominantly municipal sewage
- Date of receipt: 2012-04-19
- Storage conditions: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2-4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition for 6 days by aeration with CO2 free air until test start.
- Storage length: 6 days
- Concentration of sludge: 10 mg/L
- Initial cell/biomass concentration: 10E+7 - 10E+8 CFU/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 15 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: As prescribed by OECD TG 301B
- Solubilising agent (type and concentration if used): None
- Test temperature: 21.0 - 23.5 deg C
- pH: 7.7 - 7.9
- pH adjusted: No
- Aeration of dilution water: Yes - 30 - 100 mL/minute
- Continuous darkness: Yes - contained in brown glass bottles
TEST SYSTEM
- Culturing apparatus: Brown glass incubation bottles, 5 L capacity
- Number of culture flasks/concentration: 2 x blank control; 2 x test substance; 1 x inhibition (toxicity) control; 1 x reference substance
- Method used to create aerobic conditions: Carbon dioxide free air at 30 - 100 mL/minute flow rate
- Measuring equipment: Barium hydroxide traps
- Details of trap for CO2 and volatile organics if used: 3 serial scrubbing bottles filled with 100 mL 0.0125 mol/L barium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes. At intervals the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined by titration and used for the calculation of the produced carbon dioxide. After each sampling the other traps were moved forward and a new trap with fresh barium hydroxide solution was placed into the final position.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Toxicity control: Yes
- Other: Positive control - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 61
- Sampling time:
- 28 d
- Results with reference substance:
- 60% after 7 days, 86% after 28 days
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
- Executive summary:
Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal STP, Hildesheim, D-31137
- Reason of selection: Non adapted activated sludge from the stated sewage plant is well suited as it receives predominantly municipal sewage
- Date of receipt: 2012-04-19
- Storage conditions: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2-4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition for 6 days by aeration with CO2 free air until test start.
- Storage length: 6 days
- Concentration of sludge: 10 mg/L
- Initial cell/biomass concentration: 10E+7 - 10E+8 CFU/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: As prescribed by OECD TG 301B
- Solubilising agent (type and concentration if used): None
- Test temperature: 21.0 - 23.5 deg C
- pH: 7.7 - 7.9
- pH adjusted: No
- Aeration of dilution water: Yes - 30 - 100 mL/minute
- Continuous darkness: Yes - contained in brown glass bottles
TEST SYSTEM
- Culturing apparatus: Brown glass incubation bottles, 5 L capacity
- Number of culture flasks/concentration: 2 x blank control; 2 x test substance; 1 x inhibition (toxicity) control; 1 x reference substance
- Method used to create aerobic conditions: Carbon dioxide free air at 30 - 100 mL/minute flow rate
- Measuring equipment: Barium hydroxide traps
- Details of trap for CO2 and volatile organics if used: 3 serial scrubbing bottles filled with 100 mL 0.0125 mol/L barium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes. At intervals the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined by titration and used for the calculation of the produced carbon dioxide. After each sampling the other traps were moved forward and a new trap with fresh barium hydroxide solution was placed into the final position.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Toxicity control: Yes
- Other: Positive control - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 85
- Sampling time:
- 28 d
- Results with reference substance:
- 60% after 7 days, 86% after 28 days
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
- Executive summary:
Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal STP, Hildesheim, D-31137
- Reason of selection: Non adapted activated sludge from the stated sewage plant is well suited as it receives predominantly municipal sewage
- Date of receipt: 2012-04-19
- Storage conditions: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2-4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition for 6 days by aeration with CO2 free air until test start.
- Storage length: 6 days
- Concentration of sludge: 10 mg/L
- Initial cell/biomass concentration: 10E+7 - 10E+8 CFU/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 15 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: As prescribed by OECD TG 301B
- Solubilising agent (type and concentration if used): None
- Test temperature: 21.0 - 23.5 deg C
- pH: 7.8 - 7.9
- pH adjusted: No
- Aeration of dilution water: Yes - 30 - 100 mL/minute
- Continuous darkness: Yes - contained in brown glass bottles
TEST SYSTEM
- Culturing apparatus: Brown glass incubation bottles, 5 L capacity
- Number of culture flasks/concentration: 2 x blank control; 2 x test substance; 1 x inhibition (toxicity) control; 1 x reference substance
- Method used to create aerobic conditions: Carbon dioxide free air at 30 - 100 mL/minute flow rate
- Measuring equipment: Barium hydroxide traps
- Details of trap for CO2 and volatile organics if used: 3 serial scrubbing bottles filled with 100 mL 0.0125 mol/L barium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes. At intervals the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined by titration and used for the calculation of the produced carbon dioxide. After each sampling the other traps were moved forward and a new trap with fresh barium hydroxide solution was placed into the final position.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Toxicity control: Yes
- Other: Positive control - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 66
- Sampling time:
- 28 d
- Results with reference substance:
- 60% after 7 days, 86% after 28 days
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
- Executive summary:
Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal STP, Hildesheim, D-31137
- Reason of selection: Non adapted activated sludge from the stated sewage plant is well suited as it receives predominantly municipal sewage
- Date of receipt: 2012-04-19
- Storage conditions: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2-4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition for 6 days by aeration with CO2 free air until test start.
- Storage length: 6 days
- Concentration of sludge: 10 mg/L
- Initial cell/biomass concentration: 10E+7 - 10E+8 CFU/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 15 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: As prescribed by OECD TG 301B
- Solubilising agent (type and concentration if used): None
- Test temperature: 21.0 - 23.5 deg C
- pH: 7.7 - 7.9
- pH adjusted: No
- Aeration of dilution water: Yes - 30 - 100 mL/minute
- Continuous darkness: Yes - contained in brown glass bottles
TEST SYSTEM
- Culturing apparatus: Brown glass incubation bottles, 5 L capacity
- Number of culture flasks/concentration: 2 x blank control; 2 x test substance; 1 x inhibition (toxicity) control; 1 x reference substance
- Method used to create aerobic conditions: Carbon dioxide free air at 30 - 100 mL/minute flow rate
- Measuring equipment: Barium hydroxide traps
- Details of trap for CO2 and volatile organics if used: 3 serial scrubbing bottles filled with 100 mL 0.0125 mol/L barium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes. At intervals the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined by titration and used for the calculation of the produced carbon dioxide. After each sampling the other traps were moved forward and a new trap with fresh barium hydroxide solution was placed into the final position.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Toxicity control: Yes
- Other: Positive control - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 63
- Sampling time:
- 28 d
- Results with reference substance:
- 60% after 7 days, 86% after 28 days
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
- Executive summary:
Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal STP, Hildesheim, D-31137
- Reason of selection: Non adapted activated sludge from the stated sewage plant is well suited as it receives predominantly municipal sewage
- Date of receipt: 2012-04-19
- Storage conditions: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2-4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition for 6 days by aeration with CO2 free air until test start.
- Storage length: 6 days
- Concentration of sludge: 10 mg/L
- Initial cell/biomass concentration: 10E+7 - 10E+8 CFU/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: As prescribed by OECD TG 301B
- Solubilising agent (type and concentration if used): None
- Test temperature: 21.0 - 23.5 deg C
- pH: 7.8 - 7.9
- pH adjusted: No
- Aeration of dilution water: Yes - 30 - 100 mL/minute
- Continuous darkness: Yes - contained in brown glass bottles
TEST SYSTEM
- Culturing apparatus: Brown glass incubation bottles, 5 L capacity
- Number of culture flasks/concentration: 2 x blank control; 2 x test substance; 1 x inhibition (toxicity) control; 1 x reference substance
- Method used to create aerobic conditions: Carbon dioxide free air at 30 - 100 mL/minute flow rate
- Measuring equipment: Barium hydroxide traps
- Details of trap for CO2 and volatile organics if used: 3 serial scrubbing bottles filled with 100 mL 0.0125 mol/L barium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes. At intervals the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined by titration and used for the calculation of the produced carbon dioxide. After each sampling the other traps were moved forward and a new trap with fresh barium hydroxide solution was placed into the final position.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Toxicity control: Yes
- Other: Positive control - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 62
- Sampling time:
- 28 d
- Results with reference substance:
- 60% after 7 days, 86% after 28 days
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
- Executive summary:
Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal STP, Hildesheim, D-31137
- Reason of selection: Non adapted activated sludge from the stated sewage plant is well suited as it receives predominantly municipal sewage
- Date of receipt: 2012-04-19
- Storage conditions: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2-4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition for 6 days by aeration with CO2 free air until test start.
- Storage length: 6 days
- Concentration of sludge: 10 mg/L
- Initial cell/biomass concentration: 10E+7 - 10E+8 CFU/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 15 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: As prescribed by OECD TG 301B
- Solubilising agent (type and concentration if used): None
- Test temperature: 21.0 - 23.5 deg C
- pH: 7.7 - 7.9
- pH adjusted: No
- Aeration of dilution water: Yes - 30 - 100 mL/minute
- Continuous darkness: Yes - contained in brown glass bottles
TEST SYSTEM
- Culturing apparatus: Brown glass incubation bottles, 5 L capacity
- Number of culture flasks/concentration: 2 x blank control; 2 x test substance; 1 x inhibition (toxicity) control; 1 x reference substance
- Method used to create aerobic conditions: Carbon dioxide free air at 30 - 100 mL/minute flow rate
- Measuring equipment: Barium hydroxide traps
- Details of trap for CO2 and volatile organics if used: 3 serial scrubbing bottles filled with 100 mL 0.0125 mol/L barium hydroxide solution for the adsorption of carbon dioxide from biodegradation processes. At intervals the Total Inorganic Carbon (TIC) values of the adsorption solutions of the first trap were determined by titration and used for the calculation of the produced carbon dioxide. After each sampling the other traps were moved forward and a new trap with fresh barium hydroxide solution was placed into the final position.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Toxicity control: Yes
- Other: Positive control - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 63
- Sampling time:
- 28 d
- Results with reference substance:
- 60% after 7 days, 86% after 28 days
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
- Executive summary:
Biodegradability of the test substance has been investigated in a CO2 evolution test performed according to OECD Test Guidelines. The results obtained demonstrate that the substance is readily biodegradable.
Referenceopen allclose all
Day |
% Biodegradation |
|||
|
Positive control |
Toxicity control |
Test substance |
Test substance |
1 |
0 |
1 |
1 |
0 |
4 |
36 |
20 |
27 |
24 |
6 |
57 |
38 |
45 |
41 |
8 |
67 |
55 |
54 |
51 |
11 |
74 |
65 |
59 |
59 |
14 |
79 |
69 |
63 |
63 |
18 |
82 |
71 |
64 |
63 |
22 |
83 |
71 |
64 |
63 |
25 |
83 |
71 |
64 |
63 |
28 |
84 |
71 |
64 |
63 |
29 * |
86 |
71 |
65 |
63 |
* = Remaining 2 gas wash bottles |
||||
Day |
% Biodegradation |
|||
|
Positive control |
Toxicity control |
Test substance |
Test substance |
1 |
0 |
0 |
0 |
0 |
4 |
36 |
21 |
23 |
27 |
6 |
57 |
42 |
42 |
48 |
8 |
67 |
57 |
50 |
58 |
11 |
74 |
67 |
57 |
64 |
14 |
79 |
71 |
57 |
64 |
18 |
82 |
71 |
57 |
64 |
22 |
83 |
71 |
57 |
64 |
25 |
83 |
71 |
57 |
64 |
28 |
84 |
71 |
57 |
64 |
29 * |
86 |
71 |
57 |
64 |
* = Remaining 2 gas wash bottles |
||||
Day |
% Biodegradation |
|||
|
Positive control |
Toxicity control |
Test substance |
Test substance |
1 |
0 |
0 |
1 |
1 |
4 |
36 |
21 |
25 |
28 |
6 |
57 |
40 |
42 |
45 |
8 |
67 |
59 |
55 |
59 |
11 |
74 |
72 |
61 |
71 |
14 |
79 |
79 |
65 |
80 |
18 |
82 |
84 |
68 |
91 |
22 |
83 |
89 |
70 |
96 |
25 |
83 |
94 |
70 |
96 |
28 |
84 |
97 |
70 |
97 |
29 * |
86 |
99 |
71 |
98 |
* = Remaining 2 gas wash bottles |
||||
Day |
% Biodegradation |
|||
|
Positive control |
Toxicity control |
Test substance |
Test substance |
1 |
0 |
0 |
0 |
0 |
4 |
36 |
20 |
29 |
28 |
6 |
57 |
41 |
48 |
48 |
8 |
67 |
59 |
59 |
60 |
11 |
74 |
67 |
66 |
65 |
14 |
79 |
72 |
67 |
65 |
18 |
82 |
73 |
67 |
65 |
22 |
83 |
73 |
67 |
65 |
25 |
83 |
73 |
67 |
65 |
28 |
84 |
73 |
67 |
65 |
29 * |
86 |
73 |
67 |
65 |
* = Remaining 2 gas wash bottles |
||||
Day |
% Biodegradation |
|||
|
Positive control |
Toxicity control |
Test substance |
Test substance |
1 |
0 |
1 |
0 |
2 |
4 |
36 |
21 |
26 |
27 |
6 |
57 |
43 |
43 |
48 |
8 |
67 |
59 |
53 |
58 |
11 |
74 |
70 |
58 |
61 |
14 |
79 |
74 |
60 |
66 |
18 |
82 |
75 |
60 |
66 |
22 |
83 |
75 |
60 |
66 |
25 |
83 |
75 |
60 |
66 |
28 |
84 |
75 |
60 |
66 |
29 * |
86 |
75 |
61 |
66 |
* = Remaining 2 gas wash bottles |
||||
Day |
% Biodegradation |
|||
|
Positive control |
Toxicity control |
Test substance |
Test substance |
1 |
0 |
1 |
0 |
0 |
4 |
36 |
21 |
15 |
26 |
6 |
57 |
41 |
35 |
45 |
8 |
67 |
55 |
52 |
54 |
11 |
74 |
62 |
59 |
63 |
14 |
79 |
65 |
59 |
64 |
18 |
82 |
65 |
59 |
64 |
22 |
83 |
65 |
59 |
64 |
25 |
83 |
65 |
59 |
64 |
28 |
84 |
65 |
59 |
64 |
29 * |
86 |
65 |
59 |
64 |
* = Remaining 2 gas wash bottles |
||||
Day |
% Biodegradation |
|||
|
Positive control |
Toxicity control |
Test substance |
Test substance |
1 |
0 |
1 |
0 |
1 |
4 |
36 |
22 |
25 |
30 |
6 |
57 |
43 |
40 |
48 |
8 |
67 |
61 |
50 |
58 |
11 |
74 |
72 |
56 |
65 |
14 |
79 |
76 |
58 |
68 |
18 |
82 |
76 |
58 |
68 |
22 |
83 |
76 |
58 |
68 |
25 |
83 |
76 |
58 |
68 |
28 |
84 |
76 |
58 |
68 |
29 * |
86 |
77 |
58 |
68 |
* = Remaining 2 gas wash bottles |
||||
Description of key information
Biodegradation - Readily biodegradable
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
A number of tests of ready biodegradability have been undertaken with representative samples of sulfated oils. A technical problem during the test of the substance "oils, vegetable, sulfated, ammonium salts" occured and it was not possible to finish the test. However, for all samples tested the results obtained demonstrate that sulfated oils, analogues of the substance, are readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
