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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
23/07/2015-31/07/2015
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report Date:
2015

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Specific details on test material used for the study:
Received on 007/15 from the sponsor. It presented as a colourless translucent liquid.
Batch number: 100026324
97.5% for isomer 1 of Maderal

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Remarks:
acetone

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Source: Phytosafe s.a.r.l., 2 rue Mar Dormoy, 64000 PAU, France
Age : Less than 24h at test initiation
Fed with living celles of green algaes
Adult females were transferred in a new volume before the test initiation. Young daphnids produced within 24h were used for the tests.
Daphnids not fed during the exposure period.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Test temperature:
20.4°C and kepr constant at +/- 1°C for both range-finding and definitive test
pH:
Water control : between 7.9-7.7
Solvent control : between 7.7
Maderal : between 7.9-7.6 in all tests
Dissolved oxygen:
Water control : between 8.4-7.8 mg/L
Solvent control : between 8.4-7.7 mg/L
Maderal : between 8.4-7.6 mg/L in all tests
Nominal and measured concentrations:
Nominal concentrations:
Range finding test: 0.002 ; 0.02 ; 0.2 ; 2.1 ; 20.6 mg/L
Definitive test: 0.6 ; 1.2 ; 2.5 ; 5.1 ; 10.3 mg/L
Details on test conditions:
Test medium: M4 medium freshly reconstituted from mineral stock solutions.
Use of a Water control and a solvent control (actetone)
Tubes maintained in the dark in a climatic chamber
Reference substance (positive control):
yes
Remarks:
Potassium dichromate, CAS n°7778-50-9, AnalaR Prolabo Ref. 26784.231, batch 07J050002

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
ca. 1.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
ca. 1.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
ca. 2.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
ca. 2.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
EC50-24h value is between 0.6-2.1 mg/L. Calculations gave 1.33 mg/L as the measured value.
The results fulfilled the validity criteria base on Phytosafe historical data.
Reported statistics and error estimates:
Effect pf Maderal, 95% confidence intervals
T0+24h : 1.0-6.7 mg/L
T0+48h : 1.0-4.5 mg/L

Effect of Potassium dichromate (95% confidence intervals)
T0+24h : 0.93-1.90 mg/L

Any other information on results incl. tables

Analytical verification: test item concentrations were maintained within 80 -120% of the nominal values over the test period.

Percentage of immobilization:

Water/Solvent control: 0% in every replicates

Other observations:

Initial / final pH similar between the treatment test and both control test

Final oxygen > 3mg/L in every cases

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Executive summary:

The Short-term toxicity to aquatic invertebrates test was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test).

The results are the following:

NOEC=1.2mg/L

EC50=2.2mg/L