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EC number: 815-500-1 | CAS number: 1853175-99-1
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23/11/2015-4/12/2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1,3-dioxane, 2-(3,3-dimethyl-1-cyclohexen-1-yl)-2,5,5-trimethyl
- Cas Number:
- 1853175-99-1
- Molecular formula:
- C15H26O2
- IUPAC Name:
- 1,3-dioxane, 2-(3,3-dimethyl-1-cyclohexen-1-yl)-2,5,5-trimethyl
Constituent 1
- Specific details on test material used for the study:
- Maderal (reference ZA1076)
Batch n° 100026324
Recieved the 08/07/2015 from the sponsor
Presented as a colourless translucent liquid
Stored at at room temperature and protected from direct sun light
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- daily, in the old and new medium used
Test solutions
- Vehicle:
- yes
- Remarks:
- acetone
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Received on 10/11/2015 and previously validated
Source : Elevage de la Grande Rivière, 69490 Saint-Forgeux, France
Adaptation : maintained at 23 +/- 2°C, with a 16/8h light/dark cycle, in the same reconstitued water as used for testing
Mean lenght : measured out from 15% of the total population, taken at random : 2.6 +/- 0.2 cm
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- Range-finding test
Water control: between 21.2-22.3 °C
Solvent control: between 21.2-22.4 °C
Maderal: between: 21.1-22.6 °C
Definitive test:
Water control: between 20.1-22.1°C
Solvent control: between 20.3-22 °C
Maderal: between: 20.1-22.4 °C - pH:
- Range finding-test :
Water control : between 6.9-7.2
Solvent control : between 6.9-7.2
Maderal : between 6.9-7.2 in all tests
Definitive test :
Water control : between 7.2-7.7
Solvent control : between 7.3-7.7
Maderal : between 7.2-7.9 in all tests - Dissolved oxygen:
- Range finding-test:
Water control : between 56.8-99.7%
Solvent control : between 51.2-99.6%
Maderal : between 51.5-99.7% in all tests
Definitive test :
Water control : between 84.5-98.7%
Solvent control : between 85.9-98.7%
Maderal : between 79.6-98.7% in all tests - Nominal and measured concentrations:
- Range-finding test : 0.01 ; 0.1 ; 1.0 ; 10.0 mg a.i./L
Definitive test:
Nominal concentrations: 4.5 ; 6.3 ; 9.0 ; 12.6 ; 18.0 mg a.i/L
Measured concentrations: 2.3 ; 3.0 ; 4.1 ; 5.4 ; 5.3 mg a.i/L - Details on test conditions:
- Use of a Water control and solvent control (acetone)
Medium : ISO test water (according to ISO 6341) freshly reconstitued by dilution of mineral stock solution in pure water
Light : 16h of artificial light / d (< 500 lux)
Food : not fed during the exposure period - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 5.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 5.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- No mortality observed in water/solvent control / No mortality observed in the test item treatments, even at the highest concentration
- Results with reference substance (positive control):
- LC50-96h between 50 and 250 mg/L
Any other information on results incl. tables
- Sublethal observations / clinical signs:
The test item treatments did not remain within 80 -120% of the nominal value because of high instabiliy of the test item in water, even if the test media were alternatively renewed/adjusted twice a day. Decrease of the material was reproducible for each given concentration.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
The short-term toxicity to fish was performed according to the OECD Guideline 203 (Fish, Acute Toxicity Test).
The results are the following:
NOEC-96h = 5.4 mg a.i./L
LC50-96h > 5.4 mg a.i./L
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