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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes
Specific details on test material used for the study:
V 2870
Batch No. Eg 203
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
synthetic water prepared according to OECD guideline 202
Test organisms (species):
Daphnia magna
Details on test organisms:
laboratory breed
age of daphnia. 6 - 24 h

Holding conditions:
Temperature 18°C - 20°C
O2-Concentration at least 80 % of air saturation
pH-Value: 7.0 - 8.5
Hardness: 14° dH
Light 16 h daily
Feeding. once daily
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
18°C - 20°C
pH:
between 8.44 and 4.57 at 48 h
Dissolved oxygen:
> 60 % of air saturation
Nominal and measured concentrations:
Nominal preliminary test: 100, 10, 1 g/L, 100, 10, 1, 0.1 mg/L
Nominal main test: 100, 71, 51, 36, 26, 19, 14, 10 g/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 25 ml volumetric cylinder

- Aeration: O2-concentration > 60 % of air saturation

- No. of organisms per vessel:5
- No. of vessels per concentration (replicates):4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic water


OTHER TEST CONDITIONS
- Photoperiod: 16 h daily


VEHICLE CONTROL PERFORMED: yes

RANGE-FINDING STUDY
- Test concentrations: 100, 10, 1 g/L, 100, 10, 1, 0.1 mg/L
- Results used to determine the conditions for the definitive study: yes
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
18.62 g/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Results of the main test: The number of living daphnia (sum) and the rate of immobilisation (%) after 48 h is given.

 concentration.g/L

 100

 71

 51

 36

 26

 19

 14

 10

 control

 group I

 0

 0

 0

 0

 3

 3

 3

 4

 5

 group II

 0

 0

 0

 0

 1

 1

 4

 4

 5

 group III

 0

 0

 0  0  2  5  3  4  5
 group IV  0  0  0  3  0  3  1  5  5
 sum  0  0  0  3  6  12  11  17  20
 percentage  100  100  100  85  70  40  45  15  0
Validity criteria fulfilled:
not specified
Conclusions:
The 48 h LC0 was not determinable for the main test. The 48 h EC50 was at 18.62.g/L of the formulation "V2870".

Description of key information

The 48 h EC50 was at 18.62 g/L.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
18.62 g/L

Additional information