Trisodium [5-[[4-[(3-chloro-2-hydroxy-5-sulphophenyl)azo]-3-hydroxyphenyl]azoxy]-2-[2-(4-nitro-2-sulphophenyl)vinyl]benzenesulphonato(5-)]cuprate(3-)

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

September - December 1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards and acceptable for assessment

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

- Principle of test: equivalent or similar to a standard acute method- Parameters analysed / observed: mortality and clinical signs of intoxication

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Trisodium [5-[[4-[(3-chloro-2-hydroxy-5-sulphophenyl)azo]-3-hydroxyphenyl]azoxy]-2-[2-(4-nitro-2-sulphophenyl)vinyl]benzenesulphonato(5-)]cuprate(3-)

EC Number:

274-550-7

EC Name:

Trisodium [5-[[4-[(3-chloro-2-hydroxy-5-sulphophenyl)azo]-3-hydroxyphenyl]azoxy]-2-[2-(4-nitro-2-sulphophenyl)vinyl]benzenesulphonato(5-)]cuprate(3-)

Cas Number:

70304-38-0

Molecular formula:

UVCB

IUPAC Name:

trisodium [5-[[4-[(3-chloro-2-hydroxy-5-sulphophenyl)azo]-3-hydroxyphenyl]azoxy]-2-[2-(4-nitro-2-sulphophenyl)vinyl]benzenesulphonato(5-)]cuprate(3-)

impurity 1

Reference substance name:

Sodium sulphate

EC Number:

231-820-9

EC Name:

Sodium sulphate

Cas Number:

7757-82-6

Molecular formula:

H2O4S.2Na

IUPAC Name:

disodium sulfate

Test material form:

solid: particulate/powder

Details on test material:

SOURCE OF TEST MATERIAL- Source and lot/batch No.of test material: Saturnová hněď LB; sample No. 127/76 (No. 913)- Expiration date of the lot/batch: not specifiedPurity: 70% of the tested substance; 30% Na2SO4Mw 358.76STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL- Storage condition of test material: not specified- Solubility and stability of the test substance in the solvent/vehicle: 20 g/L of waterTREATMENT OF TEST MATERIAL PRIOR TO TESTING- Final dilution of a dissolved solid, stock liquid or gel: 20% suspension in water

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS- Source: not specified- Age at study initiation: not specified- Weight at study initiation: 115 - 150g- Fasting period before study: not specified- Housing: individually in cage - Diet (e.g. ad libitum): not specified- Water (e.g. ad libitum): not specified- Acclimation period: not specifiedENVIRONMENTAL CONDITIONSnot specified (laboratory conditions)

Administration / exposure

Route of administration:

other: unspecified, probably gavage

Vehicle:

water

Details on oral exposure:

VEHICLE - Concentration in vehicle: 20% suspension in waterMAXIMUM DOSE APPLIED: 10 g/kg

Doses:

5.02; 6.31; 7.943; 10.00 g/kg

No. of animals per sex per dose:

10

Control animals:

no

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

6 492 mg/kg bw

Based on:

test mat.

95% CL:

> 5 993 - < 7 031

Clinical signs:

strong diarrhea

Body weight:

not specified

Gross pathology:

not specified

Any other information on results incl. tables

Dose[g/kg]	Mortality
5.020	0/10
6.310	5/10
7.943	9/10
10.000	10/10

LD50 = 6.491 (5.993 – 7.031) g/kg b.w.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

very slightly toxic

Executive summary:

The test substance toxicity was evaluated on the basis of mortality and clinical signs of intoxication. According to the study results the value of LD50 of the test substance, Saturnová hněď LB, for male rats is 6.49 g/kg of body weight.