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Toxicity to soil microorganisms

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Description of key information

According to column 2 of REACH Annex IX (9.4), the study does not need to be conducted as direct and indirect exposure of the soil compartment is unlikely. Results of an aquatic microbial inhibition test showed no effect on respiration rate at a laoding of 1000 mg/L (nominal).

Key value for chemical safety assessment

Additional information

According to chemical safety assessment, the substance does not pose a risk to other compartments (water/sediment). NExBTL renewable diesel, which is a UVCB substance of similar composition to the substance, was not found to be acutely toxic to any of the aquatic species tested (algae, crustacea, fish and sediment-dwelling amphipod). Furthermore, NExBTL renewable diesel was found readily biodegradable in OECD CO2evolution test 301B.Numerous studies have also shown that alkanes are readily biodegradable in soils (Hoeppel RE et al 1991, Hawle-Ambrosch et al 2007, Miethe D et al 1994, Salanitro J 2001, Suigura et al 1996).Based on these facts, the expected soil exposure will be occasional, duration of the exposure will be short and the effects to soil organisms will be low.

References:

Hawle-Ambrosch E, Ripe W, Dornmayr-Pfaffenhuemer M, Radax C, Holzinger A and Stan-Lotter H (2007) Biodegradation of fuel oil hydrocarbons by a mixed bacterial consortium in sandy and loamy soils, Biotechnology Journal 2(12), 1564-1568.  

Hoeppel RE, Hichee RE and Arthur MF (1991) Bioventing soils contaminated with petroleum hydrocarbons.J. Indust. Microbiol. 8, 141-146  

Miethe D, Riis V and Babel W (1994) The relationship between the microbial activity of the autochthonous microorganisms of pristine and contaminated soils and their potential for the degradation of mineral oil hydrocarbons. Acta Biotechnmo. 14,131-140  

Salanitro J, 2001, Bioremediation of petroleum hydrocarbons in soil, Advances in agronomy 72, 53-105.

Sugiura K, Ishihara M, Shimauchi T and Harayama S (1997) Physicochemical properties andbiodegradabilityof crude oil Environmental Science and Technology 31(1), 45-51.