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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: well documented and scientifically acceptable

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
purity: 99.94%

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not required
Duration of treatment / exposure:
24 h
Observation period (in vivo):
1 h, 24 h, 48 h, 72 h and 7 d after application
Number of animals or in vitro replicates:
3

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
conjunctivae score
Remarks:
chemosis+redness
Basis:
mean
Time point:
24/48/72 h
Score:
5.8
Max. score:
7
Reversibility:
not fully reversible within: 7 days
Irritation parameter:
iris score
Basis:
mean
Time point:
24/48/72 h
Score:
0.77
Max. score:
2
Reversibility:
fully reversible
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
24/48/72 h
Score:
1.44
Max. score:
4
Reversibility:
not fully reversible within: 7 days
Other effects:
24 to 72 hours white, viscous discharge

Any other information on results incl. tables

Result: 3,4-dichlorphenyl isocyanate caused serious damage to eyes

   1 h   24 h   48 h   72 h   7 d
 animal #  1  2  3  1  2  3  1  2  3  1  2  3  1  2  3
 chemosis  2  3  2  3  4  4  2  3  3  2  3  3  0  2  1
 conjunctivae  2  2  2  3  3  3  3  3  3  2  3  3  0  2  1
 iris  0  0  0  1  1  1  0  1  1  0  1  1  0  0  0
 cornea  1  2  1  1  1  1  1  2  2  1  2  2  0  3  2

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye) based on GHS criteria
Conclusions:
The single apllication of the test item caused serious damage to the rabbit eye.
Executive summary:

- method: single application of 0.1 g 3,4 -dichlorophenyl isocyanate into the conjunctival sac of 3 rabbits

- result: substance is irritating to the eye (risk of serious damage to eyes)