Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 268-850-7 | CAS number: 68141-27-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01/2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Heptyl undec-10-enoate
- EC Number:
- 268-850-7
- EC Name:
- Heptyl undec-10-enoate
- Cas Number:
- 68141-27-5
- Molecular formula:
- C18H34O2
- IUPAC Name:
- heptyl undec-10-enoate
- Test material form:
- liquid
Constituent 1
Test solutions
- Details on test solutions:
- The test item was insoluble in water. Hence, the solvent medium CMC (at 30 mg/l) was used. The desired concentrations of the test upto 100 mg/l were prepared prior to the experiment, by dissolving 100 milligram of the test item with the solvent medium CMC (at 30 mg/l). 1.64 gm BG-11 medium in 1 litre demineralized water was dissolved. The pH was adjusted to 8.10 ± 0.10 using 0.1N NaOH solution.
A preliminary test was performed at four concentrations (1 mg/l, 10 mg/l, 50 mg/l & 100 mg/l) of test item.
Since, the range finding study shows significant growth inhibition in algae. The main study was performed at dose concentrations 0.1 mg/l, 0.5 mg/l, 1.0 mg/l, and 10.0 mg/l of the test item involving a comparison of responses in a control group.
Test organisms
- Test organisms (species):
- Anabaena sp.
- Details on test organisms:
- The fresh water algal strain “Anabaena sp.” was used for this study. Commercially available media for blue-green algae BG-11 was used at concentration of 1.64 gm in 1 litre demineralized Water.
A culture in test medium was prepared 4 days prior to starting the test. Initial biomass concentration of the strain ‘Anabaena sp.’ was maintained at as low as 104 cells/ml in the medium so as to allow exponential growth. The Biomass (cell count) was measured for ensuring normal growth range.
Seven days before initiation of the study pre-culture of ‘Anabaena sp.’ culture was done. The inoculated flasks were kept in the incubator room with continuous illumination of light intensity and temperature ranging from 2960 – 4440 lux and 21 to 24°C, respectively. After three days, the culture was examined under microscope to check any abnormalities or microbial contamination. During the experiment all the flasks (both control and treated) were kept in the incubator room for 72 hours continuously with above mentioned temperature and illumination
Study design
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21–24°C controlled at +/- 2 °C
- pH:
- 8.2 ± 1.5
- Nominal and measured concentrations:
- A preliminary test was performed at four concentrations (1 mg/l, 10 mg/l, 50 mg/l & 100 mg/l) of test item.
Since, the range finding study shows significant growth inhibition in algae. The main study was performed at dose concentrations 0.1 mg/l, 0.5 mg/l, 1.0 mg/l, and 10.0 mg/l of the test item involving a comparison of responses in a control group - Details on test conditions:
- Initial Biomass concentration weight : 104 cells per ml or 0.5 mg/l
Illumination : 2960- 4440 lux
Growth medium : BG-11 Medium
Apparatus to determine algal biomass : UV-Vis Spectrophotometer
No. of replicates : Five in control and three in test groups - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 8.9 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.8 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 3.6 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.1 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- Preliminary study
No growth inhibitions were recorded in ‘Heptyl Undecylenate’ at the dose concentrations of
1, 10, 50 and 100 mg/l, respectively (Table 1).
Main Study
On the basis of observations from range finding study, main study was conducted at
the concentrations 0.1 mg/l, 0.5 mg/l, 1.0 mg/l, 5.0 mg/l and 10.0 mg/l of ‘Heptyl
Undecylenate’ ’in BG-11 medium. The pH measurement in control and ‘Heptyl
Undecylenate’ concentrations are presented in Table 2. Initial pH of BG-11 medium were in
the range of 8.22 – 8.32 and the final pH were recorded in the range of 6.75-6.78 at the
test termination. The test conditions (Temperature and Light intensity of the experimental
room) during the test period are presented in Table 3. During the test, the temperature was
maintained at the range of 23.6-24.1°C and the light intensity was maintained within the
range of 4252 to 4265 lux.
The algae absorbances (optical density) in control and test item concentrations of
‘Heptyl Undecylenate’ are given in Table 4.
Percent growth inhibition of yield and specific growth rate based on control at 0-72
hours of ‘Anabaena sp.’ exposed to various concentrations of ‘Heptyl Undecylenate’ are
given in Table 5.
The maximum concentration at 0.1 mg/l of test item ‘Heptyl Undecylenate’ did not
elicit any inhibitory effects on the yield and specific growth rate of algal strain ‘Anabaena
sp.’. Therefore the effective concentrations (ECs) and no observed effect concentration
(NOEC) came out as:
EC10 : 1.8 mg/ l
EC20 : 3.6 mg/ l
EC50 : 8.9 mg/ l
NOEC : 0.1 mg/ l
The percentage inhibition and specific growth rate are calculated by comparing with
the solvent control. The percent coefficient of variation (% CV) at different time interval in
the replicate control flasks was calculated as 18.87% which is less than 35%. Hence, the
result of study complies with the requirements of the OECD Guideline.
Applicant's summary and conclusion
- Conclusions:
- Under the condition of this test, it was concluded that maximum concentration the test item ‘Heptyl Undecylenate’ at 0.1 mg/l elicited no inhibitory effects on the yield and specific growth rate of algal strain ‘Anabaena sp.’. Therefore the effective concentrations (ECs) and no observed effect concentration (NOEC) came out as:
EC10 : 1.8 mg/ l
EC20 : 3.6 mg/ l
EC50 : 8.9 mg/ l
NOEC : 0.1 mg/ l
The study was conducted as per the procedure described in the OECD Guideline for the testing of Chemicals, Freshwater Algae and Cyanobacteria, Growth Inhibition Test Guideline No. 201 Adopted on 28th July 2011. - Executive summary:
The study was conducted to determine the growth inhibition effect of ‘Heptyl Undecylenate’ on algal strain ‘Anabaena sp.’ A range finding study was conducted at four concentrations 1 mg/l, 10 mg/l, 50 mg/l & 100 mg/l of test item. Significant growth inhibitions were found at these concentrations. Therefore, on the basis of range finding study, main study was conducted using concentration of 0.1, 0.5, 1, 5 and 10 mg/l in BG-11 medium. The temperature was maintained within the range of 23.6-24.1°C and light intensity within the range of 4252-4265 Lux throughout the experimentation. Under the condition of this test, it was concluded that ‘Dicapryl Succinate’ elicited no inhibition effects at 0.1 mg/l on the yield and specific growth rate of algal strain ‘Anabaena sp’. Therefore, the Effective concentrations (ECs) and No observed effect concentration (NOEC) came out as:
EC10 : 1.8 mg/ l
EC20 : 3.6 mg/ l
EC50 : 8.9 mg/ l
NOEC : 0.1 mg/ l
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.